Library Resources for Bac End Sequencing. Final Technical Report

de Jong, Pieter J.

Health Research, Inc.

关键词: Dna;    Vectors;    59 Basic Biological Sciences;    Construction;    Awards;   

DOI  :  10.2172/772661
RP-ID  :  NONE
RP-ID  :  FG02-98ER62675
RP-ID  :  772661

美国|英语

来源: UNT Digital Library

【 摘 要 】

Studies directed towards the specific aims outlined for this research award are summarized. The RPCI II Human Bac Library has been expanded by the addition of 6.9-fold genomic coverage. This segment has been generated from a MBOI partial digest of the same anonymous donor DNA used for the rest of the library. A new cloning vector, pTARBAC1, has been constructed and used in the construction of RPCI-II segment 5. This new cloning vector provides a new strategy in identifying targeted genomic regions and will greatly facilitate a large-scale analysis for positional cloning. A new maleCS7BC/6J mouse BAC library has been constructed. RPCI-23 contain 576 plates (approx 210,000 clones) and represents approximately 11-fold coverage of the mouse genome.