THERMAL DETECTION OF DNA AND PROTEINS DURING GEL ELECTROPHORESIS

JOHNSTON, R.

Los Alamos National Laboratory

关键词: Sensitivity;    Dna;    Lanl;    59 Basic Biological Sciences;    Proteins;   

DOI  :  10.2172/768736
RP-ID  :  LA-UR-00-3985
RP-ID  :  W-7405-ENG-36
RP-ID  :  768736

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来源: UNT Digital Library

【 摘 要 】

This is the final report of a three-year, Laboratory-Directed Research and Development (LDRD) project at the Los Alamos National Laboratory (LANL). The goal of this project was to try to detect unstained, untagged, unlabeled DNA bands in real-time during gel electrophoresis using simple thermal measurements. The technical and ES&H advantages to this approach could potentially be quite significant, especially given the extreme importance of gel electrophoresis to a wide variety of practical and research fields. The project was unable to demonstrate sufficient thermal sensitivity to detect DNA bands. It is clear that we still do not understand the gel electrophoresis phenomenon very well. The temperature control techniques developed during the course of this project have other useful applications.