Hybrid Pathogen DNA Detector:Users? Manual v1.5

Schikora, B ; Hietala, S ; Shi, L ; Lee, L ; Skowronski, E ; Ardans, A

Lawrence Livermore National Laboratory

关键词: Dna;    Molecular Biology;    59 Basic Biological Sciences;    Sensitivity;    Detection;   

DOI  :  10.2172/15014078
RP-ID  :  UCRL-TR-203433
RP-ID  :  W-7405-ENG-48
RP-ID  :  15014078

美国|英语

来源: UNT Digital Library

【 摘 要 】

The Hybrid Unit uses an advanced fluidic design to move very small reagent samples through many unit operations to complete complex molecular biology experiments. The primary use of this machine is to analyze a small liquid sample for the highly specific presence of select agents known to be used in bio-warfare. The Hybrid Unit is coupled with a Luminex bead detection unit for the multiplexing of many assays in one tube. Because of this, multiple controls can be included in each run to avoid false positives. The built-in flow through PCR unit amplifies specific DNA signatures and increases sensitivity, thereby limiting exposure of handlers to highly concentrated (and potentially hazardous, spore containing) sample volumes. The reproducible precision of the Hybrid Unit also gives confidence when a signal is given that detects an agent in a given sample.