Development of a Chemoenzymatic-like and Photoswitchable Method for the Ordered Attachment of Proteins to Surfaces

Camarero, J A ; Kwon, Y ; Coleman, M

Lawrence Livermore National Laboratory

关键词: Covalence;    Surfaces;    59 Basic Biological Sciences;    Proteins;   

DOI  :  10.2172/15014684
RP-ID  :  UCRL-TR-209731
RP-ID  :  W-7405-ENG-48
RP-ID  :  15014684

美国|英语

来源: UNT Digital Library

【 摘 要 】

Protein arrays are the best tool for the rapid analysis of a whole proteome thus helping to identify all the protein/protein interactions in a living cell and they can also be used as powerful biosensors. The objective of this proposal is to develop a new entropically activated ligation method based in the naturally occurring protein trans-splicing process. This method will be used for the generation of spatially addressable arrays of multiple protein components by standard photolithographic techniques. Key to our approach is the use of the protein trans-splicing process. This naturally occurring process will allow us to create a truly generic and highly efficient method for the covalent attachment of proteins through its C-terminus to any solid support.