Autonomous Pathogen Detection System - FY02 Annual Progress Report

Colston, B ; Brown, S ; Burris, K ; Elkin, C ; Hindson, B ; Langlois, R ; Masquelier, D ; McBride, M ; Metz, T ; Nasarabadi, S ; Makarewicz, T ; Milznovich, F ; Venkateswaran, K S ; Visuri, S

Lawrence Livermore National Laboratory

关键词: Sample Preparation;    Polymerase Chain Reaction;    Toxins;    Monitoring;    59 Basic Biological Sciences;   

DOI  :  10.2172/15002220
RP-ID  :  UCRL-ID-144275-02
RP-ID  :  W-7405-ENG-48
RP-ID  :  15002220

美国|英语

来源: UNT Digital Library

【 摘 要 】

The objective of this project is to design, fabricate and field demonstrate a biological agent detection and identification capability, the Autonomous Pathogen Detector System (APDS). Integrating a flow cytometer and real-time polymerase chain reaction (PCR) detector with sample collection, sample preparation and fluidics will provide a compact, autonomously operating instrument capable of simultaneously detecting multiple pathogens and/or toxins. The APDS will operate in fixed locations, continuously monitoring air samples and automatically reporting the presence of specific biological agents. The APDS will utilize both multiplex immunoassays and nucleic acid assays to provide ''quasi-orthogonal'' multiple agent detection approaches to minimize false positives and increase the reliability of identification. Technical advances across several fronts must occur, however, to realize the full extent of the APDS. The end goal of a commercially available system for civilian biological weapon defense will be accomplished through three progressive generations of APDS instruments. The APDS is targeted for civilian applications in which the public is at high risk of exposure to covert releases of bioagent, such as major subway systems and other transportation terminals, large office complexes and convention centers. APDS is also designed to be part of a monitoring network of sensors integrated with command and control systems for wide-area monitoring of urban areas and major public gatherings. In this latter application there is potential that a fully developed APDS could add value to DoD monitoring architectures.