Autonomous pathogen detection system 2001

Langlois, R G ; Wang, A ; Colston, B ; Masquelier, D ; Jones, L ; Venkateswaran, K S ; Nasarabadi, S ; Brown, S ; Ramponi, A ; Milanovich, F P

Lawrence Livermore National Laboratory

关键词: Sample Preparation;    Pathogens;    99 General And Miscellaneous//Mathematics, Computing, And Information Science;    Monitoring;    Polymerase Chain Reaction;   

DOI  :  10.2172/15006176
RP-ID  :  UCRL-ID-142227
RP-ID  :  W-7405-ENG-48
RP-ID  :  15006176

美国|英语

来源: UNT Digital Library

【 摘 要 】

The objective of this project is to design, fabricate and field-demonstrate a fully Autonomous Pathogen Detector (identifier) System (APDS). This will be accomplished by integrating a proven flow cytometer and real-time polymerase chain reaction (PCR) detector with sample collection, sample preparation and fluidics to provide a compact, autonomously operating instrument capable of simultaneously detecting multiple pathogens and/or toxins. The APDS will be designed to operate in fixed locations, where it continuously monitors air samples and automatically reports the presence of specific biological agents. The APDS will utilize both multiplex immuno and nucleic acid assays to provide ''quasi-orthogonal'', multiple agent detection approaches to minimize false positives and increase the reliability of identification. Technical advancements across several fronts must first be made in order to realize the full extent of the APDS. Commercialization will be accomplished through three progressive generations of instruments. The APDS is targeted for domestic applications in which (1) the public is at high risk of exposure to covert releases of bioagent such as in major subway systems and other transportation terminals, large office complexes, and convention centers; and (2) as part of a monitoring network of sensors integrated with command and control systems for wide area monitoring of urban areas and major gatherings (e.g., inaugurations, Olympics, etc.). In this latter application there is potential that a fully developed APDS could add value to Defense Department monitoring architectures.