Heritable Genetic Changes in Cells Recovered From Irradiated 3D Tissue Contracts. Final report

Cornforth, Michael N.

University of Texas Medical Branch at Galveston

关键词: 63 Radiation, Thermal, And Other Environ. Pollutant Effects On Living Orgs. And Biol. Mat.;   

DOI  :  10.2172/1077983
RP-ID  :  DOE/ER--63442
RP-ID  :  FG02-02ER63442
RP-ID  :  1077983

美国|英语

来源: UNT Digital Library

【 摘 要 】

Combining contemporary cytogenetic methods with DNA CGH microarray technology and chromosome flow-sorting increases substantially the ability to resolve exchange breakpoints associated with interstitial deletions and translocations, allowing the consequences of radiation damage to be directly measured at low doses, while also providing valuable insights into molecular mechanisms of misrepair processes that, in turn, identify appropriate biophysical models of risk at low doses. The aims of this work apply to cells recovered from 3D tissue constructs of human skin and, for the purpose of comparison, the same cells irradiated in traditional 2D cultures. These aims are: to analyze by multi-flour fluorescence in situ hybridization (mFISH) the chromosomes in clonal descendents of individual human fibroblasts that were previously irradiated; to examine irradiated clones from Aim 1 for submicroscopic deletions by subjecting their DNA to comparative genomic hybridization (CGH) microarray analysis; and to flow-sort aberrant chromosomes from clones containing stable radiation-induced translocations and map the breakpoints to within an average resolution of 100 kb using the technique of 'array painting'.