Investigating the role of CheA-3 in Dusulfovibrio Vulgaris Hildenborough

Ray, Jayashee ; Keller, Kimberley ; Krierim, Bernhard ; Auer, Manfred ; Keasling, Jay ; Wall, Judy ; Mukhopadhyay, Aindrila

关键词: 12;    DESULFOVIBRIO;    ELECTRON MICROSCOPY;    GENES;    HISTIDINE;    MUTANTS;    PHENOTYPE;    PHOSPHOTRANSFERASES;    PLATES;    RECOMBINATION Desulfovibrio vulgaris Hildenborough;    chemotaxis genes;    signal transduction system;   

DOI  :  10.2172/985233
RP-ID  :  LBNL-3631E-Poster
PID  :  OSTI ID: 985233
Others  :  TRN: US1006195

美国|英语

来源: SciTech Connect

【 摘 要 】

Multiple sets of chemotaxis genes including three cheA homologs were identified in the genome sequence of the anaerobic bacterium Desulfovibrio vulgaris Hildenborough. Each CheA is a histidine kinase (HK) and part of a two component signal transduction system. Knock out mutants in the three cheA genes were created using single cross-over homologous recombination insertion. We studied the phenotypes of the cheA mutants in detail and discovered that ?cheA-3 has a non swarming/swimming phenotype both in the soft agar plates and Palleroni chamber assays. CheA-3 shows similarity to the Shewanella oneidensis CheA-3 and the Vibrio cholerae CheA-2 that are responsible for chemotaxis in the respective organisms. We did not find any morphological or structural differences between the three Delta cheA mutants and the wild type cells in electron microscopy. Our results from these studies are presented.