Next generation sequencing (NGS)technologies and applications

Vuyisich, Momchilo¹ 

[1] Los Alamos National Laboratory

关键词: AMPLIFICATION;    DATA ANALYSIS;    GENES;    NUCLEIC ACIDS;    QUALITY CONTROL;    RNA;    SYNTHESIS;   

DOI  :  10.2172/1050518
RP-ID  :  LA-UR-12-24648
PID  :  OSTI ID: 1050518
Others  :  TRN: US201218%%1040

学科分类：生物科学（综合）

美国|英语

来源: SciTech Connect

【 摘 要 】

NGS technology overview: (1) NGS library preparation - Nucleic acids extraction, Sample quality control, RNA conversion to cDNA, Addition of sequencing adapters, Quality control of library; (2) Sequencing - Clonal amplification of library fragments, (except PacBio), Sequencing by synthesis, Data output (reads and quality); and (3) Data analysis - Read mapping, Genome assembly, Gene expression, Operon structure, sRNA discovery, and Epigenetic analyses.