Development of a commercial enzymes system for lignocellulosic biomass saccharification

Kumar, Manoj

关键词: Biomass;    Enzymes;    sacchariffication;    ethanol;    lignocellulosic;    wheat straw;    biorefinery;    DSM;   

DOI  :  10.2172/1068167
RP-ID  :  DOE/GO/18079
PID  :  OSTI ID: 1068167
Others  :  Other: DSM/01

学科分类：燃料技术

美国|英语

来源: SciTech Connect

【 摘 要 】

DSM Innovation Inc., in its four year effort was able to evaluate and develop its in-house DSM fungal cellulolytic enzymes system to reach enzyme efficiency mandates set by DoE Biomass program MYPP goals. DSM enzyme cocktail is uniquely active at high temperature and acidic pH, offering many benefits and product differentiation in 2G bioethanol production. Under this project, strain and process development, ratio optimization of enzymes, protein and genetic engineering has led to multitudes of improvement in productivity and efficiency making development of a commercial enzyme system for lignocellulosic biomass saccharification viable. DSM is continuing further improvement by additional biodiversity screening, protein engineering and overexpression of enzymes to continue to further lower the cost of enzymes for saccharification of biomass.