Mutations in the Spliceosome Component prp-6 and Overexpression of cdh-5 Suppress Axon Guidance Defects of cdh-4 Mutants in Caenorhabditis elegans

Neuroscience Insights

卷:17

Original Research

Zina Aburegeba¹  Jie Pan¹  Harald Hutter¹ 

[1] Department of Biological Sciences, Simon Fraser University, Burnaby, BC, Canada

关键词: Cadherin;    central nervous system;    nervous system development;    ventral nerve cord;    axon guidance;   

DOI  :  10.1177/26331055221123346

 received in 2022-04-25, accepted in 2022-08-16,  发布年份 2022

来源: Sage Journals

【 摘 要 】

During nervous system development, axons must navigate to specific target areas. In Caenorhabditis elegans, the cadherin CDH-4 is required for ventral nerve cord axonal navigation, and dorsal nerve cord fasciculation. How CDH-4 mediates axon navigation and fasciculation is currently unknown. To identify genes acting together with cdh-4, we isolated mutants suppressing the axon guidance defects of cdh-4 mutants. These suppressors showed partial suppression of axonal defects in the dorsal and ventral nerve cords seen in cdh-4 mutants. We identified one suppressor gene, prp-6, which encodes a component of the spliceosome. Complete loss-of-function alleles of prp-6 are lethal, suggesting that the mutation isolated in our suppressor screen is a partial loss-of-function allele. A previous study found that RNAi-induced suppression of prp-6 leads to changes in the expression of several 100 genes including the cadherin cdh-5. We found that overexpression of cdh-5 mimics the suppression seen in prp-6 mutants, suggesting that CDH-5 can partially compensate for the loss of CDH-4.

【 授权许可】

CC BY-NC   
© The Author(s) 2022