Mutations in the Spliceosome Component prp-6 and Overexpression of cdh-5 Suppress Axon Guidance Defects of cdh-4 Mutants in Caenorhabditis elegans

Journal of Experimental Neuroscience

article

Zina Aburegeba¹  Jie Pan¹  Harald Hutter¹ 

[1] Department of Biological Sciences, Simon Fraser University

关键词: Cadherin;    central nervous system;    nervous system development;    ventral nerve cord;    axon guidance;   

DOI  :  10.1177/26331055221123346

学科分类：药学、药理学、毒理学（综合）

来源: Sage Journals

【 摘 要 】

During nervous system development, axons must navigate to specific target areas. In Caenorhabditis elegans , the cadherin CDH-4 is required for ventral nerve cord axonal navigation, and dorsal nerve cord fasciculation. How CDH-4 mediates axon navigation and fasciculation is currently unknown. To identify genes acting together with cdh-4 , we isolated mutants suppressing the axon guidance defects of cdh-4 mutants. These suppressors showed partial suppression of axonal defects in the dorsal and ventral nerve cords seen in cdh-4 mutants. We identified one suppressor gene, prp-6 , which encodes a component of the spliceosome. Complete loss-of-function alleles of prp-6 are lethal, suggesting that the mutation isolated in our suppressor screen is a partial loss-of-function allele. A previous study found that RNAi-induced suppression of prp-6 leads to changes in the expression of several 100 genes including the cadherin cdh-5 . We found that overexpression of cdh-5 mimics the suppression seen in prp-6 mutants, suggesting that CDH-5 can partially compensate for the loss of CDH-4.

【 授权许可】

CC BY|CC BY-NC