A modular approach to creating large engineered cartilage surfaces

JOURNAL OF BIOMECHANICS

卷:67

Article

Ford, Audrey C.¹  Chui, Wan Fung¹  Zeng, Anne Y.¹  Nandy, Aditya¹  Liebenberg, Ellen²  Carraro, Carlo³  Kazakia, Galateia⁴  Alliston, Tamara²  O'Connell, Grace D.¹ 

[1] Univ Calif Berkeley, Dept Mech Engn, 5122 Etcheverry Hall 1740, Berkeley, CA 94720 USA

[2] Univ Calif San Francisco, Dept Orthopaed Surg, San Francisco, CA 94143 USA

[3] Univ Calif Berkeley, Dept Chem Engn, Berkeley, CA 94720 USA

[4] Univ Calif San Francisco, Dept Radiol, San Francisco, CA 94143 USA

关键词: Articular cartilage;    Tissue engineering;    Modular fabrication;   

DOI  :  10.1016/j.jbiomech.2017.11.035

来源: Elsevier

【 摘 要 】

Native articular cartilage has limited capacity to repair itself from focal defects or osteoarthritis. Tissue engineering has provided a promising biological treatment strategy that is currently being evaluated in clinical trials. However, current approaches in translating these techniques to developing large engineered tissues remains a significant challenge. In this study, we present a method for developing large-scale engineered cartilage surfaces through modular fabrication. Modular Engineered Tissue Surfaces (METS) uses the well-known, but largely under-utilized self-adhesion properties of de novo tissue to create large scaffolds with nutrient channels. Compressive mechanical properties were evaluated throughout METS specimens, and the tensile mechanical strength of the bonds between attached constructs was evaluated over time. Raman spectroscopy, biochemical assays, and histology were performed to investigate matrix distribution. Results showed that by Day 14, stable connections had formed between the constructs in the METS samples. By Day 21, bonds were robust enough to form a rigid sheet and continued to increase in size and strength over time. Compressive mechanical properties and glycosaminoglycan (GAG) content of METS and individual constructs increased significantly over time. The METS technique builds on established tissue engineering accomplishments of developing constructs with GAG composition and compressive properties approaching native cartilage. This study demonstrated that modular fabrication is a viable technique for creating large-scale engineered cartilage, which can be broadly applied to many tissue engineering applications and construct geometries. (C) 2017 Elsevier Ltd. All rights reserved.

【 授权许可】

Free