Differential responses of induced pluripotent stem cell-derived cardiomyocytes to anisotropic strain depends on disease status

JOURNAL OF BIOMECHANICS

卷:48

Article

Chun, Young Wook^1,2  Voyles, David E.¹  Rath, Rutvvik¹  Hofmeister, Lucas H.¹  Boire, Timothy C.¹  Wilcox, Henry³  Lee, Jae Han¹  Bellan, Leon M.^1,4  Hong, Charles C.^2,5  Sung, Hak-Joon^1,2 

[1] Vanderbilt Univ, Dept Biomed Engn, Nashville, TN 37235 USA

[2] Vanderbilt Univ Sch Med, Div Cardiovasc Med, Nashville, TN 37232 USA

[3] Univ Tennessee, Dept Biochem & Cellular & Mol Biol, Knoxville, TN 37996 USA

[4] Vanderbilt Univ, Dept Mech Engn, Nashville, TN 37235 USA

[5] Vet Affairs TVHS, Res Med, Nashville, TN 37212 USA

关键词: Anisotropic strain;    Dilated cardiomyopathy;    IPSC;    Cardiac maturation;    Cell-cell interaction;   

DOI  :  10.1016/j.jbiomech.2015.09.028

来源: Elsevier

【 摘 要 】

Primary dilated cardiomyopathy (DCM) is a non-ischemic heart disease with impaired pumping function of the heart. In this study, we used human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) from a healthy volunteer and a primary DCM patient to investigate the impact of DCM on iPSC-CMs' responses to different types of anisotropic strain. A bioreactor system was established that generates cardiac-mimetic forces of 150 kPa at 5% anisotropic cyclic strain and 1 Hz frequency. After confirming cardiac induction of the iPSCs, it was determined that fibronectin was favorable to other extracellular matrix protein coatings (gelatin, laminin, vitronectin) in terms of viable cell area and density, and was therefore selected as the coating for further study. When iPSC-CMs were exposed to three strain conditions (no strain, 5% static strain, and 5% cyclic strain), the static strain elicited significant induction of sarcomere components in comparison to other strain conditions. However, this induction occurred only in iPSC-CMs from a healthy volunteer (control iPSC-CMs), not in iPSC-CMs from the DCM patient (DCM iPSC-CMs). The donor type also significantly influenced gene expressions of cell-cell and cell-matrix interaction markers in response to the strain conditions. Gene expression of connexin-43 (cell-cell interaction) had a higher fold change in healthy versus diseased iPSC-CMs under static and cyclic strain, as opposed to integrins alpha-5 and alpha-10 (cell-matrix interaction). In summary, our iPSC-CM-based study to model the effects of different strain conditions suggests that intrinsic, genetic-based differences in the cardiomyocyte responses to strain may influence disease manifestation in vivo. (C) 2015 Elsevier Ltd. All rights reserved.

【 授权许可】

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