被撤回的出版物: Intramuscular delivery of 3D aggregates of HUVECs and cbMSCs for cellular cardiomyoplasty in rats with myocardial infarction (Retracted article. See vol. 348, pg. 1116, 2022)

JOURNAL OF CONTROLLED RELEASE

卷:172

Article; Retracted Publication

Chen, Ding-Yuan^1,8  Wei, Hao-Ji^2,9  Lin, Wei-Wen³  Lin, Kun-Ju^4,5,6  Huang, Chieh-Cheng^1,8  Wu, Cheng-Tse^1,8  Hwang, Shiaw-Min⁷  Chang, Yen^2,9  Sung, Hsing-Wen^1,8 

[1] Natl Tsing Hua Univ, Dept Chem Engn, Hsinchu 30013, Taiwan

[2] Taichung Vet Gen Hosp, Div Cardiovasc Surg, Taipei, Taiwan

[3] Taichung Vet Gen Hosp, Div Cardiol, Taipei, Taiwan

[4] Chang Gung Univ, Dept Med Imaging & Radiol Sci, Tao Yuan, Taiwan

[5] Chang Gung Mem Hosp, Dept Nucl Med, Linkou, Taiwan

[6] Chang Gung Mem Hosp, Mol Imaging Ctr, Linkou, Taiwan

[7] Food Ind Res & Dev Inst, Bioresource Collect & Res Ctr, Hsinchu, Taiwan

[8] Natl Tsing Hua Univ, Inst Biomed Engn, Hsinchu, Taiwan

[9] Natl Yang Ming Univ, Coll Med, Taipei 112, Taiwan

关键词: Myocardial infarction;    Cell-based therapy;    Cell delivery;    Neovascularization;    Tissue engineering;   

DOI  :  10.1016/j.jconrel.2013.06.025

来源: Elsevier

【 摘 要 】

Cell-based therapeutic neovascularization is a promising method for treating ischemic disorders. In this work, human umbilical vein endothelial cells (HUVECs) were thoroughly premixed with cord-blood mesenchymal stem cells (cbMSCs) and cultivated to form three-dimensional (3D) cell aggregates for cellular cardiomyoplasty. In the in vitro study, tubular networks were formed at day 1 after the co-culturing of dissociated HUVECs and cbMSCs on Matrigel; however, as time progressed, the grown tubular networks regressed severely. Conversely, when 3D cell aggregates were grown on Matrigel, mature and stable tubular networks were observed over time, under the influence of their intensive cell-extracellular matrix (ECM) interactions and cell-cell contacts. 3D cell aggregates were transplanted into the peri-infarct zones of rats with myocardial infarction (MI) via direct intramyocardial injection. Based on our pinhole single photon emission computed tomography (SPECT) myocardial-perfusion observations, echocardiographic heart-function examinations and histological analyses, the engrafted 3D cell aggregates considerably enhanced the vascular densities and the blood flow recovery in the ischemic myocardium over those of their dissociated counterparts, thereby reducing the size of perfusion defects and restoring cardiac function. These results demonstrate that the intramuscular delivery of 3D cell aggregates of HUVECs/cbMSCs can be a valuable cell-based regenerative therapeutic strategy against MI. (C) 2013 Elsevier B.V. All rights reserved.

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