Dose sparing enabled by skin immunization with influenza virus-like particle vaccine using microneedles

JOURNAL OF CONTROLLED RELEASE

卷:147

Article

Quan, Fu-Shi^1,2  Kim, Yeu-Chun^1,2,3  Compans, Richard W.^1,2  Prausnitz, Mark R.³  Kang, Sang-Moo^1,2 

[1] Emory Univ, Sch Med, Dept Microbiol & Immunol, Atlanta, GA 30322 USA

[2] Emory Univ, Sch Med, Yerkes Vaccine Ctr, Atlanta, GA 30322 USA

[3] Georgia Inst Technol, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA

关键词: Influenza;    Microneedle;    Virus-like particle vaccine;    Dose sparing;    Skin;   

DOI  :  10.1016/j.jconrel.2010.07.125

来源: Elsevier

【 摘 要 】

To address the limitations of conventional influenza vaccine manufacturing and delivery, this study investigated administration of virus-like particle (VLP) influenza vaccine using a microneedle patch. The goal was to determine if skin immunization with influenza VLP vaccine using microneedles enables dose sparing. We found that low-dose influenza (A/PR/8/34 H1N1) VLP vaccination using microneedles was more immunogenic than low-dose intramuscular (IM) vaccination and similarly immunogenic as high-dose IM vaccination in a mouse model. With a 1 mu g dose of vaccine, both routes showed similar immune responses and protective efficacy, with microneedle vaccination being more effective in inducing recall antibody responses in lungs and antibody secreting cells in bone marrow. With a low dose of vaccine (0.3 mu g), microneedle vaccination induced significantly superior protective immunity, which included binding and functional antibodies as well as complete protection against a high dose lethal infection with A/PR/8/34 virus, whereas IM immunization provided only partial (40%) protection. Therefore, this study demonstrates that microneedle vaccination in the skin confers more effective protective immunity at a lower dose, thus providing vaccine dose-sparing effects. (C) 2010 Elsevier B.V. All rights reserved.

【 授权许可】

Free