Large-scale production of foot-and-mouth disease virus (serotype Asia1) VLP vaccine in Escherichia coli and protection potency evaluation in cattle

BMC Biotechnology

Research Article

Yuxin Huang¹  Chaochao Lv¹  Yuzhou Wang¹  Xiangdong Li¹  Yan Xiao¹  He Yan¹  Wenqiang Pang¹  Yajie Xuan¹  Kegong Tian²  Hong-Ying Chen³  Bo Yin³  Y. Adam Yuan⁴  Xiaobing Mo⁴ 

[1] National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, 471003, Luoyang, People’s Republic of China;National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, 471003, Luoyang, People’s Republic of China;College of Animal Science and Veterinary Medicine, Henan Agricultural University, 450002, Zhengzhou, Henan, People’s Republic of China;National University of Singapore (Suzhou) Research Institute, 377 Lin Quan Street, 215123, Suzhou Industrial Park, Jiangsu, China;National University of Singapore (Suzhou) Research Institute, 377 Lin Quan Street, 215123, Suzhou Industrial Park, Jiangsu, China;Department of Biological Sciences and Centre for Bioimaging Sciences, National University of Singapore, 14 Science Drive 4, 117543, Singapore, Singapore;

关键词: Foot-and-mouth disease;    Serotype Asia 1;    Virus-like particle vaccine;    Fifty percent protection dose (PD);    E. coli;   

DOI  :  10.1186/s12896-016-0285-6

 received in 2016-01-21, accepted in 2016-06-21,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundFoot-and-mouth disease (FMD) is an acute, highly contagious disease that infects cloven-hoofed animals. Vaccination is an effective means of preventing and controlling FMD. Compared to conventional inactivated FMDV vaccines, the format of FMDV virus-like particles (VLPs) as a non-replicating particulate vaccine candidate is a promising alternative.ResultsIn this study, we have developed a co-expression system in E. coli, which drove the expression of FMDV capsid proteins (VP0, VP1, and VP3) in tandem by a single plasmid. The co-expressed FMDV capsid proteins (VP0, VP1, and VP3) were produced in large scale by fermentation at 10 L scale and the chromatographic purified capsid proteins were auto-assembled as VLPs in vitro. Cattle vaccinated with a single dose of the subunit vaccine, comprising in vitro assembled FMDV VLP and adjuvant, developed FMDV-specific antibody response (ELISA antibodies and neutralizing antibodies) with the persistent period of 6 months. Moreover, cattle vaccinated with the subunit vaccine showed the high protection potency with the 50 % bovine protective dose (PD50) reaching 11.75 PD50 per dose.ConclusionsOur data strongly suggest that in vitro assembled recombinant FMDV VLPs produced from E. coli could function as a potent FMDV vaccine candidate against FMDV Asia1 infection. Furthermore, the robust protein expression and purification approaches described here could lead to the development of industrial level large-scale production of E. coli-based VLPs against FMDV infections with different serotypes.

【 授权许可】

CC BY   
© The Author(s). 2016