Affinity-based growth factor delivery using biodegradable, photocrosslinked heparin-alginate hydrogels

JOURNAL OF CONTROLLED RELEASE

卷:154

Article

Jeon, Oju¹  Powell, Caitlin¹  Solorio, Loran D.¹  Krebs, Melissa D.¹  Alsberg, Eben^1,2 

[1] Case Western Reserve Univ, Dept Biomed Engn, Cleveland, OH 44106 USA

[2] Case Western Reserve Univ, Dept Orthopaed Surg, Cleveland, OH 44106 USA

关键词: Alginate hydrogel;    Controlled release;    Heparin;    Affinity binding;    BMP-2;    Bone formation;   

DOI  :  10.1016/j.jconrel.2011.06.027

来源: Elsevier

【 摘 要 】

Photocrosslinkable biomaterials are promising for tissue engineering applications due to their capacity to be injected and form hydrogels in situ in a minimally invasive manner. Our group recently reported on the development of photocrosslinked alginate hydrogels with controlled biodegradation rates, mechanical properties, and cell adhesive properties. In this study, we present an affinity-based growth factor delivery system by incorporating heparin into photocrosslinkable alginate hydrogels (HP-ALG), which allows for controlled, prolonged release of therapeutic proteins. Heparin modification had minimal effect on the biodegradation profiles, swelling ratios, and elastic moduli of the hydrogels in media. The release profiles of growth factors from this affinity-based platform were sustained for 3 weeks with no initial burst release, and the released growth factors retained their biological activity. Implantation of bone morphogenetic protein-2 (BMP-2)-loaded photocrosslinked alginate hydrogels induced moderate bone formation around the implant periphery. Importantly. BMP-2-loaded photocrosslinked HP-ALG hydrogels induced significantly more osteogenesis than BMP-2-loaded photocrosslinked unmodified alginate hydrogels, with 1.9-fold greater peripheral bone formation and 1.3-fold greater calcium content in the BMP-2-loaded photocrosslinked HP-ALG hydrogels compared to the BMP-2-loaded photocrosslinked unmodified alginate hydrogels after 8 weeks implantation. This sustained and controllable growth factor delivery system, with independently controllable physical and cell adhesive properties, may provide a powerful modality for a variety of therapeutic applications. (C) 2011 Elsevier B.V. All rights reserved.

【 授权许可】

Free