Fast spatial-selective delivery into live cells

JOURNAL OF CONTROLLED RELEASE

卷:266

Article

Xiong, Ranhua^1,2  Drullion, Claire³  Verstraelen, Peter⁴  Demeester, Jo¹  Skirtach, Andre G.^2,5  Abbadie, Corinne³  De Vos, Winnok H.^2,4,5  De Smedt, Stefaan C.^1,6  Braeckmans, Kevin^1,2,7,8 

[1] Univ Ghent, Fac Pharmaceut Sci, Lab Gen Biochem & Phys Pharm, B-9000 Ghent, Belgium

[2] Univ Ghent, Fac Pharmaceut Sci, Ctr Nano & Biophoton, B-9000 Ghent, Belgium

[3] Univ Lille, CNRS, Inst Pasteur Lille, UMRMech Tumorigenesis & Targeted Therapies 8161 M, F-59000 Lille, France

[4] Univ Antwerp, Dept Vet Sci, B-2020 Antwerp, Belgium

[5] Univ Ghent, Dept Mol Biotechnol, B-9000 Ghent, Belgium

[6] NFU, Coll Chem Engn, Jiangsu Key Lab Biomass Based Green Fuels & Chem, Nanjing 210037, Jiangsu, Peoples R China

[7] Univ Lille 1, Univ Lille Nord France, IEMN, UMR 8520, F-59652 Villeneuve Dascq, France

[8] Univ Lille 1, Univ Lille Nord France, Lab Phys Lasers Atomes & Mol, UMR 8523, F-59655 Villeneuve Dascq, France

关键词: Intracellular delivery;    Cell-selective delivery;    Nanoparticles;    Pulsed laser;    Vapour nanobubbles;   

DOI  :  10.1016/j.jconrel.2017.09.033

来源: Elsevier

【 摘 要 】

Intracellular delivery of functional compounds into living cells is of great importance for cell biology as well as therapeutic applications. Often it is sufficient that the compound of interest (being a molecule or nanoparticle) is delivered to the cell population as a whole. However, there are applications that would benefit considerably from the possibility of delivering a compound to a certain subpopulation of cells, or even in selected single cells. Here we report on an integrated platform for high-throughput spatially resolved nanoparticle-enhanced photoporation (SNAP) of adherent cells. SNAP enables safe, intracellular delivery of exogenously administered nanomaterials in selected subpopulations of cells, even down to the single cell level. We demonstrate the power of SNAP by selectively delivering a safe contrast agent into a subpopulation of polynucleated keratinocytes, enabling their downstream purification for unraveling their role in neoplasm formation. The flexibility and speed with which individual cells can be labeled make SNAP an ideal tool for high-throughput applications, not only for selective labeling but also for targeted drug delivery.

【 授权许可】

Free