Airway epithelial cells from asthmatic children differentially express proremodeling factors

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:129

Article

Lopez-Guisa, Jesus M.¹  Powers, Claire¹  File, Daniele¹  Cochrane, Elizabeth²  Jimenez, Nathalia³  Debley, Jason S.^1,2 

[1] Univ Washington, Ctr Tissue & Cell Sci, Seattle Childrens Res Inst, Seattle, WA 98195 USA

[2] Univ Washington, Seattle Childrens Hosp, Div Pulm Med, Seattle, WA 98195 USA

[3] Univ Washington, Seattle Childrens Hosp, Dept Anesthesia & Pain Management, Seattle, WA 98195 USA

关键词: Asthma;    children;    airway remodeling;    epithelial cells vascular endothelial growth factor;    a disintegrin and metalloprotease 33;    periostin;    TGF-beta 2;   

DOI  :  10.1016/j.jaci.2011.11.035

来源: Elsevier

【 摘 要 】

Background: The airway epithelium can express factors that drive subepithelial airway remodeling. TGF-beta 2, vascular epithelial growth factor (VEGF), a disintegrin and metalloprotease 33 (ADAM33), and periostin are hypothesized to be involved in subepithelial remodeling and are overexpressed in adult asthmatic airways. Epidemiologic data suggest that lung function deficits in asthmatic patients are acquired in childhood. Objectives: We sought to determine whether airway epithelial cells (AECs) from asthmatic children differentially express TGF-beta 2, VEGF, ADAM33, or periostin compared with cells from atopic nonasthmatic and healthy children intrinsically or in response to IL-4/IL-13 stimulation. Methods: Bronchial and nasal epithelial cells were obtained from brushings from well-characterized asthmatic (n = 16), atopic nonasthmatic (n = 9), and healthy (n = 15) children after achievement of anesthesia for elective procedures. After differentiation at an air-liquid interface (ALI) for 3 weeks, conditioned media were sampled and RNA was extracted from unstimulated and IL-4/IL-13-stimulated cultures. TGF-beta 2 and VEGF levels were measured with ELISA. ADAM33 and periostin expression was assessed by using real-time PCR. Results: TGF-beta 2 and VEGF production was significantly greater in bronchial and nasal ALI cultures from asthmatic children than in cultures from atopic nonasthmatic and healthy children. TGF-beta 2 levels increased significantly in asthmatic cultures after IL-4/IL-13 stimulation. Within-subject correlation between nasal and bronchial ALI production of TGF-beta 2 (r = 0.64, P = .001) and VEGF (r = 0.73, P < .001) was good. Periostin expression was 3.7-fold higher in bronchial cells (P < .001) and 3.9-fold higher in nasal cells (P < .004) from asthmatic children than in cells from atopic nonasthmatic or healthy children. ADAM33 was not differentially expressed by AECs from asthmatic patients compared with that from cells from atopic nonasthmatic or healthy children. Conclusion: AECs from asthmatic children differentially express TGF-beta 2, VEGF, and periostin compared with cells from atopic nonasthmatic and healthy children. Nasal epithelial cells might be a suitable surrogate for bronchial cells that could facilitate investigation of the airway epithelium in future longitudinal pediatric studies. (J Allergy Clin Immunol 2012;129:990-7.)

【 授权许可】

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