Genetic polymorphisms in arginase I and II and childhood asthma and atopy

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:117

Article

Li, HL ; Romieu, I ; Sienra-Monge, JJ ; Ramirez-Aguilar, M ; del Rio-Navarro, BE ; Kistner, EO ; Gjessing, HK ; Lara-Sanchez, ID ; Chiu, GY ; London, SJ

关键词: ARG1;    ARG2;    genetic predisposition to disease;    SNP;    polymorphism;    single nucleotide;    respiratory hypersensitivity;    skin tests;    asthma;    tobacco smoke pollution;   

DOI  :  10.1016/j.jaci.2005.09.026

来源: Elsevier

【 摘 要 】

Background: A recent microarray study implicated arginase I (ARGI) and arginase II (ARG2) in mouse allergic asthma models and human asthma. Objectives: To examine the association between genetic variation in ARGI and ARG2 and childhood asthma and atopy risk. Methods: We enrolled 433 case-parent triads, consisting of patients with asthma 4 to 17 years old and their biologic parents, from the allergy clinic of a public hospital in Mexico City between 1998 and 2003. Atopy to 24 aeroallergens was determined by skin prick tests. We genotyped 4 single nucleotide polymorphisms (SNPs) of ARG1 and 4 SNPs of ARG2 with minor allele frequencies higher than 10% by using the TaqMan assay (Roche Molecular Systems, Pleasanton, Calif). Results: ARGI SNPs and haplotypes were not associated with asthma, but all 4 ARGI SNPs were associated with the number of positive skin tests (P = .007-.018). Carrying 2 copies of minor alleles for either of 2 highly associated ARG2 SNPs was associated with a statistically significant increased relative risk (RR) of asthma (1.5, 95% CI = 1.1-2.1 for arg2s1; RR = 1.6, 95% CI = 1.1-2.3 for arg2s2). The association was slightly stronger among children with a smoking parent (arg2s1 RR 2.1, 95% CI = 1.2 - 3.9 with a smoking parent; RR = 1.2, 95% CI = 0.8-1.9 without; interaction P = .025). Haplotype analyses reduced the sample size but supported the single SNP results. One ARG2 SNP was related to the number of positive skin tests (P = .027).

【 授权许可】

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