Targeting IL-4/IL-13 signaling to alleviate oral allergen-induced diarrhea

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:123

Article

Orekov, Tatyana¹  Finkelman, Fred D.^1,3  Rothenberg, Marc E.² 

[1] Cincinnati Childrens Hosp, Med Ctr, Div Immunobiol, Cincinnati, OH 45229 USA

[2] Cincinnati Childrens Hosp, Med Ctr, Dept Pediat, Div Allergy & Immunol, Cincinnati, OH 45229 USA

[3] Univ Cincinnati, Coll Med, Div Immunol, Cincinnati, OH 45221 USA

关键词: Allergy;    anaphylaxis;    IL-4;    IL-13;    IL-13R alpha 1;    intestine;    mast cell;   

DOI  :  10.1016/j.jaci.2008.10.001

来源: Elsevier

【 摘 要 】

Background: Intestinal anaphylaxis (manifested by acute diarrhea) is dependent on IgE and mast cells. Objective: We aimed to define the respective roles of IL-4 and IL-13 and their receptors in disease pathogenesis. Methods: Wild-type mice and mice deficient in IL-4, IL-43, and IL-13 receptor (IL-13R) alpha 1 (part of the type 2 IL-4 receptor [11,411]) were sensitized with ovalbumin (OVA)/aluminum potassium sulfate and subsequently given repeated intragastric OVA exposures. The IL-4R alpha chain was targeted with anti IL-4R alpha mAb before or after intragastric OVA exposures. Results: IL4(-/-) (and IL4/IL3(-/-)) mice produced almost no IgE and were highly resistant to OVA-induced diarrhea, whereas allergic diarrhea was only partially impaired in IL13(-/-) and IL13R alpha 1(-/-) mice. IL13R alpha 1-deficient mice had decreased IgE levels, despite having normal baseline IL-4 levels. Intestinal mast cell accumulation and activation also depended mainly on IL-4 and, to a lesser extent, on IL-13. Prophylactic anti-IL4R alpha mAb treatment, which blocks all IL-4 and IL-13 signaling, suppressed development of allergic diarrhea. However, treatment with anti-IL-4R alpha mAb for 7 days only partially suppressed IgE and did not prevent intestinal diarrhea. Conclusion: Endogenously produced IL-13 supplements the ability of IL-4 to induce allergic diarrhea by promoting oral allergen sensitization rather than the effector phase of intestinal anaphylaxis. (J Allergy Clin Immunol 2009;123:53-8.)

【 授权许可】

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