Nasal IL-4+CXCR5+CD4+ T follicular helper cell counts correlate with local IgE production in eosinophilic nasal polyps

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:137

Article

Zhang, Ya-Na^1,3  Song, Jia¹  Wang, Hai¹  Wang, Heng¹  Zeng, Ming¹  Zhai, Guan-Ting¹  Ma, Jin¹  Li, Zhi-Yong¹  Liao, Bo¹  Wang, Bao-Feng¹  Zhen, Zhen^1,4  Wang, Nan¹  Cao, Ping-Ping¹  Lin, Peng³  Ning, Qin²  Liu, Zheng¹ 

[1] Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Dept Otolaryngol Head & Neck Surg, 1095 Jiefang Ave, Wuhan 430030, Peoples R China

[2] Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Dept Infect Dis,Inst Infect Dis, Wuhan 430030, Peoples R China

[3] Tianjin First Ctr Hosp, Dept Otolaryngol Head & Neck Surg, Tianjin, Peoples R China

[4] Peking Univ, Hosp 1, Dept Otolaryngol Head & Neck Surg, Beijing 100871, Peoples R China

关键词: B cell;    ectopic lymphoid structure;    eosinophil;    IgE;    IL-4;    nasal polyp;    T follicular helper cell;   

DOI  :  10.1016/j.jaci.2015.07.025

来源: Elsevier

【 摘 要 】

Background: Locally produced IgE contributes to the initiation and development of eosinophilic inflammation in eosinophilic nasal polyps independent of systemic atopy. However, whether CXCR5(+)CD4(+) T follicular helper (T-FH) cells are involved in local IgE production at mucosal sites remains unexplored. Objective: We sought to explore the presence, phenotype, and function of CXCR5(+)CD4(+) T-FH cells in eosinophilic nasal polyp tissues compared with noneosinophilic nasal polyp and control normal nasal tissues. Methods: T-FH cell-surface phenotypes and subsets and B-cell subsets in nasal tissues and peripheral blood were studied by means of flow cytometry. Immunohistochemistry was used to detect the tissue location of T-FH cells. Sorted nasal T-FH cells and CXCR5(-) T cells were cultured with autologous naive B cells purified from blood. Results: Nasal T-FH cells expressed inducible costimulator, programmed cell death protein 1, and the transcription factor B-cell lymphoma 6 (Bcl-6) at an intermediate level when compared with bona fide T-FH cells in tonsils and circulating T-FH cells. Although counts of total T-FH cells and IL-21(+), IFN-gamma(+), and IL-17(+) T-FH cells were increased in both eosinophilic and noneosinophilic nasal polyp tissues compared with those in normal nasal tissues, IL-4(+) T-FH cell counts were only increased in eosinophilic polyp tissues. IL-4 and IL-21 were involved in polyp T-FH cell-induced IgE production from naive B cells, and nasal IL-4(+) T-FH cell counts correlated highly with local IgE levels in vivo. IL-4(+)Bcl-6(+)CD4(+) T-FH cells were identified in ectopic lymphoid structures in eosinophilic nasal polyps. T-FH cells also positively correlated with germinal center B cells and plasma cells in nasal tissues. Conclusion: Nasal IL-4(+) T-FH cells might be involved in local IgE production in eosinophilic nasal polyps.

【 授权许可】

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