Impairment of T-regulatory cells in cord blood of atopic mothers

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:121

Article

Schaub, Bianca¹  Liu, Jing^1,3  Hoeppler, Sabine¹  Haug, Severine¹  Sattler, Christine²  Lluis, Anna¹  Illi, Sabina¹  von Mutius, Erika¹ 

[1] Univ Children Hosp Munich, Dr Von Haunerschen Kinderspital, Dept Pulm & Allergy, D-80337 Munich, Germany

[2] Univ Munich, Univ Hosp, Dept Gynecol, Munich, Germany

[3] Jilin Univ, Hosp 2, Dept Resp Med, Changchun 130023, Peoples R China

关键词: adaptive;    cord blood;    cytokines;    innate;    IL;    regulatory T cells;    T(H)17 cells;    Toll-like receptor;   

DOI  :  10.1016/j.jaci.2008.04.010

来源: Elsevier

【 摘 要 】

Background: Maternal atopy is a strong predictor for the development of childhood allergic diseases. The underlying mechanisms are ill defined, yet regulatory T (Treg) and T(H)17 cells may play a key role potentially shaping the early immune system toward a proallergic or antiallergic immune regulation. Objective: We examined T(H)1/T(H)2, Treg, and T(H)17 cell responses to innate (lipid A/peptidoglycan) and mitogen/adaptive (phytohemagglutinin/Dermatophagoides pteronyssinus 1) immune stimulation in cord blood from offspring of atopic/nonatopic mothers. Methods: Cord blood mononuclear cells from 161 healthy neonates (59% nonatopic, 41% atopic mothers) were investigated regarding Treg and T(H)17 cells (mRNA/surface markers), suppressive function, and proliferation/cytokine secretion. Results: Cord blood from offspring of atopic mothers showed fewer innate-induced Treg cells (CD4(+)CD25(+) high), lower mRNA expression of associated markers (glucocorticoid-induced tumor necrosis factor receptor-related protein/lymphocyte activation gene 3; P < .05), and a trend toward lower Forkhead box transcription factor 3 (Foxp3) expression. Treg cell function was impaired in mitogen-induced suppression of T effector cells in cord blood of offspring from atopic mothers (P = .03). Furthermore, IL-10 and IFN-gamma secretion were decreased in innate-stimulated cord blood of offspring from atopic mothers (P = .04/.05). Innate-induced IL-17 was independent of maternal atopy and highly correlated with IL-13 secretion. Conclusion: In offspring of atopic mothers, Treg cell numbers, expression, and function were impaired at birth. T(H)17 cells were correlated with T(H)2 cells, independently of maternal atopy.

【 授权许可】

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