Cytokine response after severe respiratory syncytial virus bronchiolitis in early life

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:122

Article

Castro, Mario¹  Schweiger, Toni¹  Yin-DeClue, Huiquing¹  Ramkumar, Thiruvamoor P.¹  Christie, Chandrika¹  Zheng, Jie³  Cohen, Rebecca¹  Schechtman, Kenneth B.³  Strunk, Robert²  Bacharier, Leonard B.² 

[1] Washington Univ, Sch Med, Dept Internal Med, Div Pulm & Crit Care Med, St Louis, MO 63110 USA

[2] Washington Univ, Sch Med, Dept Pediat, St Louis, MO 63110 USA

[3] Washington Univ, Sch Med, Dept Biostat, St Louis, MO 63110 USA

关键词: RSV bronchiolitis;    asthma;    eczema;    allergic sensitization;    cytokines;   

DOI  :  10.1016/j.jaci.2008.07.010

来源: Elsevier

【 摘 要 】

Background: Immune response after viral infection usually involves T(H)1-mediated response; however, severe respiratory syncytial virus (RSV) infection appears to be associated with the development of asthma, a T(H)2-predominant phenotype. Objective: To understand the early and subsequent immunologic response to a serious RSV infection in children over time. Methods: A total of 206 previously healthy infants hospitalized with severe RSV bronchiolitis were enrolled in a prospective cohort called the RSV Bronchiolitis in Early Life study. Peripheral blood T cells were obtained immediately after RSV infection and at 2, 4, and 6 years of age, stimulated with phorbol 12-myristate 13-acetate and ionomycin, and analyzed for IL-2, IL-4, IL-13, and IFN-gamma by flow cytometry and real-time PCR. Results: Of the children, 48% (n = 97) developed asthma (physician-diagnosed), and 48% (n = 97) had eczema by age 6 years; 32% (n = 48 of 150) developed allergic sensitization by 3 years of age. Children with asthma had lower IL-13 expression at 6 years of age than those without (P = .001). IFN-gamma, IL-2, and IL-4 levels did not differ by asthma or eczema status during follow-up (all P > .05). Allergic sensitization was not associated with differences in cytokine levels during follow-up (all P > .05). Conclusion: Severe RSV infection early in life is associated with a high incidence of asthma and eczema. Contrary to expectations, subsequent immunologic development in those who developed asthma, eczema, or allergic sensitization was not associated with a T(H)2 phenotype in the peripheral blood.

【 授权许可】

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