Characterization of the allergic T-cell response to Pru p 3, the nonspecific lipid transfer protein in peach

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:124

Article

Schulten, Veronique¹  Radakovics, Astrid¹  Hartz, Christina²  Mari, Adriano³  Vazquez-Cortes, Sonia⁴  Fernandez-Rivas, Montserrat⁴  Lauer, Iris²  Jahn-Schmid, Beatrice¹  Eiwegger, Thomas⁵  Scheurer, Stephan²  Bohle, Barbara^1,6 

[1] Med Univ Vienna, Ctr Physiol Pathophysiol & Immunol, Dept Pathophysiol, A-1090 Vienna, Austria

[2] Paul Ehrlich Inst, D-6070 Langen, Germany

[3] IDI IRCCS, Ctr Clin & Expt Allergol, Rome, Italy

[4] Hosp Clin San Carlos, Serv Alergia, Madrid, Spain

[5] Med Univ Vienna, Dept Pediat, A-1090 Vienna, Austria

[6] Christian Doppler Lab Immunomodulat, Vienna, Austria

关键词: Peach allergy;    food allergy;    nonspecific lipid transfer protein;    Pru p 3;    T cells;    T(H)1/T(H)2;    IL-10;   

DOI  :  10.1016/j.jaci.2009.02.010

来源: Elsevier

【 摘 要 】

Background: Pru p 3, the nonspecific lipid transfer protein from peach, is an important plant food allergen that frequently induces systemic reactions. Objective: We sought to analyze the allergic T-cell response to Pru p 3. Methods: PBMCs from Italian and Spanish patients with peach allergy were stimulated with purified natural Pru p 3. Allergen-specific T-cell lines were used to identify T-cell epitopes of Pru p 3. Pru p 3-specific T-cell clones (TCCs) were analyzed for allergen-induced secretion of IL-4, IIFN-gamma, and EL-10 and expression of the integrin 07, a receptor critical for gut homing. Results: No difference in T-cell responses of Italian and Spanish patients was found. Among several T cell-activating regions, Pru p 3(13-27), Pru p 3(34-48), Pru p 3(43-57), and Pru p 3(61-75) were most frequently recognized in 18 Pro p 3-specific T-cell lines. The majority of 32 Pru p 3-specific TCCs belonged to the T(H)2 subset. In contrast to TCCs specific for other plant food and pollen allergens, only a limited number of Pru p 3-specific TCCs produced significant amounts of IL-10. The expression of integrin beta 7 on Pru p 3-specific TCCs was comparable with that observed on peanut-specific TCCs and higher compared with that seen in different pollen-specific TCCs. Conclusion: The T-cell response to Pru p 3 is dominated by TH2 cells presumably primed in the gut. The identification of relevant T cell-activating regions provides a basis for engineering hypoallergenic variants of Pru p 3 with less IgE binding and retained T-cell stimulatory capacity for safe immunotherapy of peach allergy. (J Allergy Clin Immunol 2009;17,4:100-7.)

【 授权许可】

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