Impact of Glutathione on the Allergenicity of the Peach Lipid Transfer Protein Pru p 3

Journal of Investigational Allergology and Clinical Immunology

Jensen-Jarolim E¹  Díaz-Perales A⁴  Roth-Walter F³  Cuesta-Herranz J²  Starkl P³  Kinkel J³  Tordesillas L⁴  Gómez-Casado C³ 

[1] Institute of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology and Immunology, Medical University Vienna, Austria;Allergy Service, Jimenez Diaz Foundation, Madrid, Spain;Comparative Medicine, Messerli Research Institute, University of Veterinary Medicine Vienna, Medical University Vienna and University of Vienna, Vienna, Austria;Centre for Plant Genomics and Biotechnology (UPM-INIA), Pozuelo de Alarcón, Spain

关键词: IgE-binding;    Reducing agent;    Peach allergy;    Pru p 3;    GSH;   

Others  :  1183631

【 摘 要 】

Background and Objective: The allergenic potential of proteins can be altered under various physicochemical conditions. Glutathione (GSH) is a reducing agent that is used as an antioxidant in food products. We aimed to characterize the natural folding of peach proteins and test the allergenicity of reduced and natural Pru p 3, the major peach allergen.

Methods: Pru p 3 was purified from peach, and its conformation was analyzed by means of circular dichroism. Using a thiol fluorescent probe, reduced proteins were detected in fresh peach. GSH-reduced Pru p 3 was tested in vitro for T-cell proliferation and in vivo using skin prick testing.

Results: GSH-reduced Pru p 3 produced variable skin prick reactions in peach-allergic patients. The proliferative response of peripheral blood mononuclear cells from allergic patients to reduced Pru p 3 tended to be less intense, whereas secretion of the cytokines IFN-γ, IL-5, and IL-10 was comparable. In a pool of sera from peach-allergic patients, reduction hardly impaired IgE-binding. Moreover, the stability of reduced Pru p 3 to gastrointestinal digestion was similar to that of the natural form.

Conclusions: GSH can at least transiently reduce Pru p 3. We found that the effect of reduction on the allergenicity of Pru p 3 varied. Therefore, as an additive, GSH does not seem to eliminate the risk of reactions for peach-allergic patients.