MicroRNA

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:141

Article

Lu, Thomas X.¹  Rothenberg, Marc E.² 

[1] Univ Chicago, Div Gastroenterol, Chicago, IL 60637 USA

[2] Cincinnati Childrens Hosp Med Ctr, Div Allergy & Immunol, Cincinnati, OH 45229 USA

关键词: Allergy;    antagomiRs;    antimiRs;    CRISPR;    CRISPR/Cas9;    gene silencing;    in situ hybridization;    microarray;    microRNA;    microRNA methods;    miRNA;    miRNA expression;    miRNA inhibitors;    miRNA isolation;    miRNA mimics;    miRNA profiling;    miRNA quantitation;    miRNA targets;    qPCR;    stem-loop;   

DOI  :  10.1016/j.jaci.2017.08.034

来源: Elsevier

【 摘 要 】

MicroRNAs (miRNAs) are small endogenous RNAs that regulate gene-expression posttranscriptionally. MiRNA research in allergy is expanding because miRNAs are crucial regulators of gene expression and promising candidates for biomarker development. MiRNA mimics and miRNA inhibitors currently in preclinical development have shown promise as novel therapeutic agents. Multiple technological platforms have been developed for miRNA isolation, miRNA quantitation, miRNA profiling, miRNA target detection, and modulating miRNA levels in vitro and in vivo. Here we will review the major technological platforms with consideration given for the advantages and disadvantages of each platform.

【 授权许可】

Free