Allergen immunotherapy induces a suppressive memory response mediated by IL-10 in a mouse asthma model

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:113

Article

Vissers, JLM ; van Esch, BCAM ; Hofman, GA ; Kapsenberg, ML ; Weller, FR ; van Oosterhout, AJM

关键词: asthma;    allergy;    immunotherapy;    IL-10;    IgE;    hyper-responsiveness;    eosinophilia;    T(H)2 lymphocytes;    regulatory T cells;    suppression;   

DOI  :  10.1016/j.jaci.2004.02.041

来源: Elsevier

【 摘 要 】

Background: Human studies have demonstrated that allergen immunotherapy induces memory suppressive responses and IL-10 production by allergen-specific T cells. Previously, we established a mouse model in which allergen immunotherapy was effective in the suppression of allergen-induced asthma manifestations. Objective: In this study, we examined whether immunotherapy induces a long-lasting effect and investigated the role of IL-10 in successful immunotherapy. Methods: Ovalbumin-sensitized BALB/c mice were treated with 3 injections of ovalbumin (1 mg, subcutaneous) on alternate days. After a short interval (1 week) and after a long interval (5 weeks), mice were challenged by ovalbumin inhalation, and subsequently, airway reactivity, airway eosinophilia, ovalbumin-specific IgE, and T(H)2 cytokine profile were measured. Flow cytometry and blocking of IL-10 receptors in vivo were used to gain insight in the role of IL-10 in the beneficial effects of allergen immunotherapy. Results: After a long interval between ovalbumin immunotherapy and ovalbumin challenge, the development of airway eosinophilia and hyperresponsiveness to methacholine were as strongly suppressed as after a short interval. These suppressive effects coincided with significantly reduced serum ovalbumin-specific IgE levels and T(H)2 cytokine production. On immunotherapy, the IL-5:IL-10 ratio in the bronchoalveolar lavage fluid shifted toward IL-10. In ovalbumin-restimulated lung cell and thoracic lymph node cultures from these mice, IL-5 levels dramatically decreased, whereas the percentage of IL-10(+)CD4(+) T cells was not affected. Finally, in mice treated with mAb against IL-10 receptors, the beneficial effects of immunotherapy were largely abrogated. Conclusion: These data demonstrate that allergen immunotherapy induces a memory suppressive effect in which IL-10 is essential.

【 授权许可】

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