Bullous pemphigoid outcome is associated with CXCL10-induced matrix metalloproteinase 9 secretion from monocytes and neutrophils but not lymphocytes

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:139

Article

Riani, Meriem¹  Le Jan, Sebastien¹  Plee, Julie^1,2  Durlach, Anne⁴  Le Naour, Richard³  Haegeman, Guy⁵  Bernard, Philippe^1,2  Antonicelli, Frank^1,2 

[1] Univ Hosp, Derm I C EA7319, Dermatol Lab, Reims, France

[2] Univ Hosp, Dept Dermatol, Reims, France

[3] Univ Reims, Lab IMAB, EA4683, Reims, France

[4] Univ Hosp, Hosp Maison Blanche, Lab Pol Bouin, Reims, France

[5] Univ Ghent, LEGEST, Ghent, Belgium

关键词: Bullous pemphigoid;    autoimmunity;    inflammation;    CXCL10;    selective glucocorticoid receptor activator;    compound A;   

DOI  :  10.1016/j.jaci.2016.08.012

来源: Elsevier

【 摘 要 】

Background: The outcome of bullous pemphigoid (BP), the most frequent autoimmune skin-blistering disease, involves matrix metalloproteinase 9 (MMP-9), IL-17, and IL-23 release from infiltrated inflammatory cells. The chemokine CXCL10 has been associated with several autoimmune diseases, but its participation in BP pathophysiology still needs to be clarified. Objective: We sought to assess whether BP outcome was associated with different CXCL10 levels and to evaluate the contribution of CXCL10 to the described cytokine/protease inflammatory loop associated with disease outcome. Methods: Skin biopsy specimens (n = 16), serum (n 5 114), blister fluid (n 5 23), and primary inflammatory cells from patients with BP were used to investigate CXCL10 expression and function. Results: At baseline, both resident cells, such as keratinocytes and fibroblasts, and infiltrating immune cells expressed CXCL10 at lesional sites in skin of patients with BP. CXCL10 levels were higher in blister fluid (P <.0001) and serum (P <.005) from patients with BP than in serum from age- and sex-matched control subjects (n 5 34). Furthermore, CXCL10 serum levels increased at day 60 only in patients who relapsed within the first year of treatment (n 5 33, P <.005). Interestingly, CXCL10 expression could be upregulated by itself and IL-17 in inflammatory cells. Notably, neutrophils and monocytes from patients with BP, but not lymphocytes, responded to CXCL10 by increasing MMP-9 secretion through the activation of extracellular signal-regulated kinase 1/2, p38, phosphoinositide-3 kinase signaling pathways. Finally, CXCL10-increased MMP-9 secretion was inhibited by methylprednisolone and also by compound A, a novel nonsteroidal glucocorticoid receptor ligand. Conclusion: We showed that increased levels of inflammatory biomarkers in patients with BP, such as CXCL10, favor neutrophil- and monocyte-associated MMP-9 release and disease relapse and opened new therapeutic horizons in patients with this autoimmune disease.

【 授权许可】

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