Implications of mycoremediated dry olive residue application and arbuscular mycorrhizal fungi inoculation on the microbial community composition and functionality in a metal-polluted soil

JOURNAL OF ENVIRONMENTAL MANAGEMENT

卷:247

Article

Garcia-Sanchez, Mercedes^1,2  Cajthaml, Tomas^3,4  Filipova, Alena^3,4  Tlustos, Pavel¹  Szakova, Jirina¹  Garcia-Romera, Inmaculada⁵ 

[1] Fac Agrobiol Food & Nat Resources, Dept Agroenvironm Chem & Plant Nutr, CZ-129 Prague 6, Czech Republic

[2] INRA, UMR Eco & Sols, 2 Pl Viala, F-34060 Montpellier 2, France

[3] Acad Sci Czech Republ, Vvi, Inst Microbiol, Videnska 1083, CZ-14220 Prague 4, Czech Republic

[4] Charles Univ Prague, Fac Sci, Inst Environm Studies, Benatska 2, CZ-12801 Prague 2, Czech Republic

[5] CSIC, EEZ, Dept Soil Microbiol & Symbiot Syst, C Prof Albareda 1, Granada 18001, Spain

关键词: Funneliformis mosseae;    Metal-pollution;    Microbial activities;    mycoremediated dry olive residue (MDOR);    Phospholipid fatty acids (PLFAs);    Soil restoration;   

DOI  :  10.1016/j.jenvman.2019.05.101

来源: Elsevier

【 摘 要 】

Metal-polluted soils represent hostile environments affecting the composition and functions of soil microbial communities. This study evaluated the implication of combining the mycoremediated dry olive residue (MDOR) amendment application with the inoculation of the arbuscular mycorrhizal fungi (AMF) Fwineliformis mosseae in restoring the quality, composition, and functionality of soil microbial communities. To achieve this aim, a mesocosms experiment was set up that included three variations: i) with and without application of Penicilliwn chrysogenum-10-transformed MDOR (MDOR_Pc), and Chondrosterwn pwpureum-transformed MDOR (MDOR_Cp) amendments; ii) with and without F. mosseae inoculation; and iii) 30-day and 60-day soil treatment time. As a result of this combined treatment, changes in the soil labile organic C and N fractions were observed throughout the experiment. Increases in the abundance of phospholipid fatty acids (PLFAs) for bacteria, actinobacteria, and Gram and Gram + bacteria were also recorded at the end of the experiment. The addition of MDOR amendments boosted fungal and AM fungi communities. AM fungi root and soil colonization was also enhanced as the result of improvement nutrient turnover and spatial conditions caused by adding MDOR in combination with an inoculation of F. mosseae. The composition and functionality of microbial communities seemed to be an important ecological attribute indicating an apparently fully functional restoration of this metal-polluted soil and therefore suggesting the suitability of the combined MDOR and AM fungus treatment as a reclamation practice.

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