Development of a loop-mediated isothermal amplification method for the rapid detection of the dioxin-degrading bacterium Ochrobactrum anthropi in soil

JOURNAL OF ENVIRONMENTAL MANAGEMENT

卷:160

Article

Chen, Hsi-Jien¹  Lai, Jun-Yu¹  Lee, Meng-Shiou² 

[1] Ming Chi Univ Technol, Dept Safety Hlth & Environm Engn, New Taipei City 24301, Taiwan

[2] China Med Univ, Coll Pharm, Grad Inst Pharmaceut Chem, Taichung 40402, Taiwan

关键词: LAMP;    Loop-mediated isothermal amplification;    Dioxin;    Biomonitoring;    Ochrobactrum anthropi BD-1;   

DOI  :  10.1016/j.jenvman.2015.06.027

来源: Elsevier

【 摘 要 】

In this study, loop-mediated isothermal amplification (LAMP) and real-time LAMP assays were developed to detect the dioxin-degrading bacterium Ochrobactrum anthropi strain BD-1 in soil. Four primers were designed to use ITS gene amplification for the strain O. anthropi BD-1. The real-time LAMP assay was found to accomplish the reaction by 1 pg of genomic DNA load when used for nucleic acid amplification. This assay was then applied to detect O. anthropi BD-1 in eight soil samples collected from a diox-incontaminated site. The results demonstrated that these newly developed LAMP and real-time LAMP assays will not only be useful and efficient tools for detecting the target gene, but also be used as molecular tools for monitoring the growth of dioxin-degrading O. anthropi in the soil. This is the first report to demonstrate the use of LAMP assays to monitor the presence of O. anthropi in dioxin-contaminated soil. The application of this method should improve the biomonitoring of dioxin contamination. (C) 2015 Elsevier Ltd. All rights reserved.

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