Detection of nisin and fibrinogen adsorption on poly(ethylene oxide) coated polyurethane surfaces by time-of-flight secondary ion mass spectrometry (TOF-SIMS)

JOURNAL OF COLLOID AND INTERFACE SCIENCE

卷:358

Article

Schilke, Karl F.¹  McGuire, Joseph¹ 

[1] Oregon State Univ, Sch Chem Biol & Environm Engn, Corvallis, OR 97331 USA

关键词: Nisin;    Fibrinogen;    TOF-SIMS;    Protein adsorption;    Medical polymers;    PEO-polybutadiene-PEO triblock surfactants;    Robust PCA;   

DOI  :  10.1016/j.jcis.2011.03.014

来源: Elsevier

【 摘 要 】

Stable, pendant polyethylene oxide (PEO) layers were formed on medical-grade Pellethane (R) and Tygon (R) polyurethane surfaces, by adsorption and gamma-irradiation of PEO-polybutadiene-PEO triblock surfactants. Coated and uncoated polyurethanes were challenged individually or sequentially with nisin (a small polypeptide with antimicrobial activity) and/or fibrinogen, and then analyzed with time-of-flight secondary ion mass spectrometry (TOF-SIMS). Data reduction by robust principal components analysis (PCA) allowed detection of outliers, and distinguished adsorbed nisin and fibrinogen. Fibrinogen-contacted surfaces, with or without nisin, were very similar on uncoated polymer surfaces, consistent with nearly complete displacement or coverage of previously-adsorbed nisin by fibrinogen. In contrast, nisin-loaded PEO layers remained essentially unchanged upon challenge with fibrinogen, suggesting that the adsorbed nisin is stabilized within the pendant PEO layer, while the peptide-loaded PEO layer retains its ability to repel large proteins. Coatings of PEO loaded with therapeutic polypeptides on medical polymers have the potential to be used to produce anti-fouling and biofunctional surfaces for implantable or blood-contacting devices. (c) 2011 Elsevier Inc. All rights reserved.

【 授权许可】

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