A methacrylate-based polymeric imidazole ligand yields quantum dots with low cytotoxicity and low nonspecific binding

JOURNAL OF COLLOID AND INTERFACE SCIENCE

卷:458

Article

Johnson, Colin M.¹  Pate, Kayla M.²  Shen, Yi¹  Viswanath, Anand¹  Tan, Rui¹  Benicewicz, Brian C.^1,3  Moss, Melissa A.^2,4  Greytak, Andrew B.^1,3 

[1] Univ S Carolina, Dept Chem & Biochem, Columbia, SC 29208 USA

[2] Univ S Carolina, Dept Chem Engn, Columbia, SC 29208 USA

[3] Univ S Carolina, USC Nanoctr, Columbia, SC 29208 USA

[4] Univ S Carolina, Biomed Engn Program, Columbia, SC 29208 USA

关键词: CdSe;    Core/shell quantum dots;    Bio-imaging;    Toxicity;    Nonspecific binding;    Fluorescence microscopy;    Live cells;    Ligand exchange;    Polymer;    Gel permeation chromatography;   

DOI  :  10.1016/j.jcis.2015.07.069

来源: Elsevier

【 摘 要 】

This paper assesses the biocompatibility for fluorescence imaging of colloidal nanocrystal quantum dots (QDs) coated with a recently-developed multiply-binding methacrylate-based polymeric imidazole ligand. The QD samples were purified prior to ligand exchange via a highly repeatable gel permeation chromatography (GPC) method. A multi-well plate based protocol was used to characterize nonspecific binding and toxicity of the QDs toward human endothelial cells. Nonspecific binding in 1% fetal bovine serum was negligible compared to anionically-stabilized QD controls, and no significant toxicity was detected on 24 h exposure. The nonspecific binding results were confirmed by fluorescence microscopy. This study is the first evaluation of biocompatibility in QDs initially purified by GPC and represents a scalable approach to comparison among nanocrystal-based bioimaging scaffolds. (C) 2015 Elsevier Inc. All rights reserved.

【 授权许可】

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