Effect of the ultrastructure of chitosan nanoparticles in colloidal stability, quorum quenching and antibacterial activities

JOURNAL OF COLLOID AND INTERFACE SCIENCE

卷:556

Article

Vila-Sanjurjo, C.^1,3  David, L.²  Remunan-Lopez, C.³  Vila-Sanjurjo, A.⁴  Goycoolea, F. M.^1,5 

[1] Westfalische Wilhelms Univ Munster, IBBP, Lab Nanobiotechnol, Schlosspl 8, D-48143 Munster, Germany

[2] Univ Claude Bernard Lyon 1, Univ Lyon, CNRS, UMR 5223,Ingn Mat Polymeres IMP Lyon 1, 15 Bd A Latarjet, F-69622 Villeurbanne, France

[3] Univ Santiago De Compostela, Dept Pharm & Pharmaceut Technol, Campus Vida S-N, Santiago De Compostela 15782, Spain

[4] Univ A Coruna, CICA, Dept Biol Celular & Mol, Grp GIBE, Campus Zapateira S-N, Coruna 15071 A, Spain

[5] Univ Leeds, Sch Food Sci & Nutr, Leeds LS2 9JT, W Yorkshire, England

关键词: Chitosan;    Nanoparticles;    Ionic gelation;    Genipin;    Quorum sensing;    Quorum quenching;    Antimicrobial;    Acyl-homoserine lactone;    Biosensor;    Synchrotron SAXS;    Crosslinking;    E. coli;   

DOI  :  10.1016/j.jcis.2019.08.061

来源: Elsevier

【 摘 要 】

We have fabricated two types of crosslinked chitosan-based nanoparticles (NPs), namely (1) ionically crosslinked with tripolyphosphate (TPP), designated as IC-NPs and (2) dually co-crosslinked (ionically and covalently with TPP and genipin, respectively) termed CC-NPs. The two types of NPs were physichochemically characterized by means of DLS-NIBS, synchrotron SAXS and M3-PALS (zeta potential). First, we found that covalent co-crosslinking of ionically pre-crosslinked nanoparticles yielded monodisperse CC-NPs in the size range of similar to 200 nm, whereas the parental IC-NPs remained highly polydisperse. While both types of chitosan nanoparticles displayed a core-shell structure, as determined by synchrotron SAXS, only the structure of CC-NPs remained stable at long incubation times. This enhanced structural robustness of CC-NPs was likely responsible of their superior colloidal stability even in biological medium. Second, we explored the antimicrobial and quorum sensing inhibition activity of both types of nanoparticles. We found that CC-NPs had lower long-term toxicity than IC-NPs. In contrast, sub-lethal doses of IC-NPs consistently displayed higher levels of quorum quenching activity than CC-NPs. Thus, this work underscores the influence of the NP's ultrastructure on their colloidal and biological properties. While the cellular and molecular mechanisms at play are yet to be fully elucidated, our results broaden the spectrum of use of chitosan-based nanobiomaterialsin the development of antibiotic-free approaches against Gram-negative pathogenic bacteria. (C) 2019 Elsevier Inc. All rights reserved.

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