Poly-L-lysine/heparin multilayer coatings prevent blood protein adsorption

JOURNAL OF COLLOID AND INTERFACE SCIENCE

卷:485

Article

Barrantes, Alejandro^1,2  Wengenroth, Jonas¹  Arnebrant, Thomas²  Haugen, Havard J.¹ 

[1] Univ Oslo, Dept Biomat, Inst Clin Dent, POB 1109 Blindern, N-0317 Oslo, Norway

[2] Malmo Univ, Biomed Sci Hlth & Soc, S-20560 Malmo, Sweden

关键词: Layer by layer;    Protein adsorption;    Ellipsometry;    QCM-D;    Heparin coating;   

DOI  :  10.1016/j.jcis.2016.09.046

来源: Elsevier

【 摘 要 】

The adsorption of blood proteins, serum albumin (BSA), immunoglobulin G (IgG) and fibrinogen (FGN), onto model SiO2 planar surfaces coated with poly-L-lysine/heparin multilayers (PLL/HEP) has been investigated by means of ellipsometry and quartz crystal microbalance with dissipation. Aiming at the development of low fouling coatings, this study has been focused on the effects that the number of layers and the type of polyelectrolyte present on the topmost layer have on the adsorption of these proteins. The three proteins interact with PLL-ended coatings whereas HEP-ended coatings prevent the adsorption of both BSA and IgG and induce a decrease in the adsorbed amount of FGN, down to 0.4 mg/m(2) for three bilayers, as the number of PLL/HEP bilayers increases. These results suggest that heparin-ended multilayers prevent protein adsorption, which is an indicative of good blood compatibility. As a consequence we propose that PLL/HEP coatings could be used for the development of vascular medical devices. (C) 2016 Elsevier Inc. All rights reserved.

【 授权许可】

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