Selective patterning of Si-based biosensor surfaces using isotropic silicon etchants

JOURNAL OF COLLOID AND INTERFACE SCIENCE

卷:369

Article

Biggs, Bradley W.¹  Hunt, Heather K.^1,2  Armani, Andrea M.^1,3 

[1] Univ So Calif, Mork Family Dept Chem Engn & Mat Sci, Los Angeles, CA 90089 USA

[2] Univ Missouri, Dept Biol Engn, Columbia, MO 65211 USA

[3] Univ So Calif, Ming Hsieh Dept Elect Engn Electrophys, Los Angeles, CA 90089 USA

关键词: Biological sensors;    Surface immobilization;    Bioconjugation;   

DOI  :  10.1016/j.jcis.2011.11.082

来源: Elsevier

【 摘 要 】

Ultra-sensitive, label-free biosensors have the potential to have a tremendous impact on fields like medical diagnostics. For the majority of these Si-based integrated devices, it is necessary to functionalize the surface with a targeting ligand in order to perform specific biodetection. To do this, silane coupling agents are commonly used to immobilize the targeting ligand. However, this method typically results in the bioconjugation of the entire device surface, which is undesirable. To compensate for this effect, researchers have developed complex blocking strategies that result in selective patterning of the sensor surface. Recently, silane coupling agents were used to attach biomolecules to the surface of silica toroidal biosensors integrated on a silicon wafer. Interestingly, only the silica biosensor surface was conjugated. Here, we hypothesize why this selective patterning occurred. Specifically, the silicon etchant (xenon difluoride), which is used in the fabrication of the biosensor, appears to reduce the efficiency of the silane coupling attachment to the underlying silicon wafer. These results will enable future researchers to more easily control the bioconjugation of their sensor surfaces, thus improving biosensor device performance. (C) 2011 Elsevier Inc. All rights reserved.

【 授权许可】

Free