Cobalt and Titanium nanoparticles influence on human osteoblast mitochondrial activity and biophysical properties of their cytoskeleton

JOURNAL OF COLLOID AND INTERFACE SCIENCE

卷:531

Article

Perni, Stefano¹  Yang, Lirong¹  Preedy, Emily Callard¹  Prokopovich, Polina¹ 

[1] Cardiff Univ, Sch Pharm & Pharmaceut Sci, Cardiff CF10 3NB, S Glam, Wales

关键词: Osteoblast;    Cobalt;    Titanium;    Nanoparticles;    Wear debris;   

DOI  :  10.1016/j.jcis.2018.07.028

来源: Elsevier

【 摘 要 】

We investigated the biophysical effects (cell elasticity and spring constant) caused on Saos-2 human osteoblast-like cells by nanosized metal (Co and Ti) wear debris, as well as the adhesive characteristics of cells after exposure to the metal nanoparticles. Cell mitochondria! activity was investigated using the MIT assays; along with LDH assay, metal uptake, cell apoptosis and mineralisation output (alizarin red assay) of the cells. Osteoblasts mitochondrial activity was not affected by Ti nanoparticles at concentrations up to 1 mg/ml and by Cobalt nanoparticles at concentrations < 0.5 mg/ml; however elasticity and spring constant were significantly modified by the exposure to nanoparticles of these metals in agreement with the alteration of cell conformation (shape), as result of the exposure to simulated wear debris, demonstrated by fluorescence images after actin staining. (C) 2018 Elsevier Inc. All rights reserved.

【 授权许可】

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