DETECTION OF HEPATITIS-C VIRUS-ANTIBODIES WITH NEW RECOMBINANT ANTIGENS - ASSESSMENT IN CHRONIC LIVER-DISEASES

JOURNAL OF HEPATOLOGY

卷:15

Article

RIEZUBOJ, JI ; PARKER, D ; CIVEIRA, MP ; PHIPPARD, D ; CORBISHLEY, TP ; CAMPS, J ; CASTILLA, A ; PRIETO, J

关键词: HCV ANTIBODY;    POLYMERASE CHAIN REACTION;    CHRONIC HEPATITIS-C;    CHRONIC HEPATITIS-B;    ALCOHOLIC LIVER DISEASE;    PRIMARY BILIARY CIRRHOSIS;    HEPATOCELLULAR CARCINOMA;   

DOI  :  10.1016/0168-8278(92)90061-S

来源: Elsevier

【 摘 要 】

A new serological assay to detect antibodies against hepatitis C, based on a recombinant protein (BHC10) which incorporates structural and non-structural viral antigens, was tested in 67 healthy subjects and 409 patients with various forms of liver disease. Results were compared with the current assay based on the recombinant non-structural viral antigen c100 and with the recently introduced second-generation assay, Ortho2. None of the healthy subjects was positive by any of the assays. In patients with chronic non-A, non-B hepatitis the prevalence of anti-BHC10 was 96.8%, higher than anti-c100 (83.3%, p < 0.001) and similar to Ortho2 (94.3%). False-positive results were less frequently found when BHC10 was used. These findings show that assays incorporating structural and non-structural antigens provide higher sensitivity to detect hepatitis C virus infection and they define an almost exclusive role of hepatitis C virus in the genesis of chronic non-A, non-B hepatitis.

【 授权许可】

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