Augmentation of chloramphenicol degradation by Geobacter-based biocatalysis and electric field

JOURNAL OF HAZARDOUS MATERIALS

卷:410

Article

Xiao, Leilei^1,2  Li, Jiajia^1,2  Lichtfouse, Eric^4,5  Li, Zhenkai⁶  Wang, Quan⁶  Liu, Fanghua^1,2,3 

[1] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China

[2] Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China

[3] Guangdong Acad Sci, Natl Reg Joint Engn Res Ctr Soil Pollut Control &, Guangdong Key Lab Integrated Agroenvironm Pollut, Inst Ecoenvironm & Soil Sci, Guangzhou 510650, Peoples R China

[4] Aix Marseille Univ, Coll France, CNRS, IRD,INRAE,CEREGE, Ave Louis Philibert, F-13100 Aix En Provence, France

[5] Xi An Jiao Tong Univ, State Key Lab Multiphase Flow Power Engn, Xian 710049, Shaanxi, Peoples R China

[6] Ludong Univ, Sch Resources & Environm Engn, Yantai 264025, Peoples R China

关键词: Chloramphenicol removal;    Geobacter;    Electric filed;    Critical voltage;    Synergistic operation;   

DOI  :  10.1016/j.jhazmat.2020.124977

来源: Elsevier

【 摘 要 】

Electroactive microorganisms and electrochemical technologies have been separately used for environmental remediation such as antibiotics removal, yet the efficiency of coupling these two methods for chlorinated antibiotics removal is poorly known. Here we tested the synergy of Geobacter sulfurreducens PCA, an electroactive bacteria, and an electrical field, on chloramphenicol removal. Removal is increased two-fold by increasing the temperature from 30?C to 37?C. The cyclic voltammograms and chronoamperometry tests demonstrated that G. sulfurreducens PCA catalyzed chloramphenicol chemical reduction with electrode as excusive electron donor. A critical voltage, -0.6 to -0.5 V vs. Ag/AgCl, was discovered for chloramphenicol degradation with an increase of removal rate about 2.62-folds, from 31.06% to 81.41%. Combined removal with both G. sulfurreducens PCA and an electrical field increased the apparent rate constant and reached 82.77% removal at -0.5 V. Specially, the combined removal at -0.5 V even presented more robust removal efficiency compared to -0.6 V (78.64%) without G. sulfurreducens PCA. Mass spectrometry of degradation products indicates the reduction of nitro into amine groups, and dechlorination into less toxic compounds. Overall, combined biocatalysis and an electrical field is a promising method to remove antibiotics from polluted environments.

【 授权许可】

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