Enhanced dechlorination of 1,2-dichloropropane to propene in a bioelectrochemical system mediated by Dehalogenimonas

JOURNAL OF HAZARDOUS MATERIALS

卷:416

Article

Fernandez-Verdejo, David¹  Cortes, Pilar²  Blanquez, Paqui¹  Marco-Urrea, Ernest¹  Guisasola, Albert³ 

[1] Univ Autonoma Barcelona, Biorem UAB, Dept Chem Biol & Environm Engn, Sch Engn, Barcelona 08193, Spain

[2] Univ Autonoma Barcelona, Fac BioCiencies, Dept Genet & Microbiol, Barcelona 08193, Spain

[3] Univ Autonoma Barcelona, GENOCOV, Dept Chem Biol & Environm Engn, Sch Engn, Barcelona 08193, Spain

关键词: Dehalogenimonas;    1, 2-dichloropropane;    Bioelectrochemistry;    Remediation;    Organohalide respiring bacteria;   

DOI  :  10.1016/j.jhazmat.2021.126234

来源: Elsevier

【 摘 要 】

Bioelectrochemical systems (BES) are promising technologies to enhance the growth of organohalide-respiring bacteria and to treat chlorinated aliphatic hydrocarbons. In this study, two carbon-based cathodic electrode materials, a graphite brush and a carbon cloth, were used as hydrogen suppliers to couple growth of Dehalogenimonas and dechlorination of 1,2-DCP to nontoxic propene in the cathode vessel. The BES with graphite brush electrode consumed similar to 4000 mu M 1,2-DCP during 110 days and exhibited a degradation rate 5.6-fold higher than the maximum value obtained with the carbon cloth electrode, with a cathode potential set at -0.7 V. Quantitative PCR confirmed that Dehalogenimonas gene copies increased by two orders of magnitude in the graphite brush BES, with an average yield of 1.2.10(8)+/- 5.10(7) cells per mu mol of 1,2-DCP degraded. The use of a pulsed voltage operation (cathode potential set at -0.6 V for 16 h and -1.1 V for 8 h) increased the coulombic efficiency and degradation of 1,2-DCP when compared with a continuous voltage operation of -1.1 V. Bacterial cell aggregates were observed in the surface of the graphite brush electrodes by electron scanning microscopy, suggesting biofilm formation. This study expands the range of chlorinated compounds degradable and organohalide-respiring bacteria capable of growing in BES.

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