Detoxification of 1,1,2-trichloroethane to ethene in a bioreactor co-culture of Dehalogenimonas and Dehalococcoides mccartyi strains

JOURNAL OF HAZARDOUS MATERIALS

卷:331

Article

Hatijah Mortan, Siti¹  Martin-Gonzalez, Lucia¹  Vicenta, Teresa¹  Caminal, Gloria²  Nijenhuis, Ivonne³  Adrian, Lorenz³  Marco-Urrea, Ernest¹ 

[1] Univ Autonoma Barcelona, Dept Engn Quim Biol & Ambiental, Barcelona, Spain

[2] CSIC, Inst Quim Avancada Catalunya IQAC, Barcelona, Spain

[3] UFZ Helmholtz Ctr Environm Res, Dept Isotope Biogeochem, Leipzig, Germany

关键词: Organohalide-respiring bacteria;    Fed-batch reactor;    Dehalococcoides;    Dehalogenimonas;    1,1,2-Trichloroethane;   

DOI  :  10.1016/j.jhazmat.2017.02.043

来源: Elsevier

【 摘 要 】

1,1,2-Trichloroethane (1,1,2-TCA) is a non-flammable organic solvent and common environmental contaminant in groundwater. Organohalide-respiring bacteria are key microorganisms to remediate 1,1,2-TCA because they can gain metabolic energy during its dechlorination under anaerobic conditions. However, all current isolates produce hazardous end products such as vinyl chloride, monochloroethane or 1,2-dichloroethane that accumulate in the medium. Here, we constructed a syntrophic co-culture of Dehalogenimonas and Dehalococcoides mccartyi strains to achieve complete detoxification of 1,1,2-TCA to ethene. In this co-culture, Dehalogenimonas transformed 1,1,2-TCA via dihaloelimination to vinyl chloride, whereas Dehalococcoides reduced vinyl chloride via hydrogenolysis to ethene. Molasses, pyruvate, and lactate supported full dechlorination of 1,1,2-TCA in serum bottle co-cultures. Scale up of the cultivation to a 5-L bioreactor operating for 76 din fed-batch mode was successful with pyruvate as substrate. This synthetic combination of bacteria with known complementary metabolic capabilities demonstrates the potential environmental relevance of microbial cooperation to detoxify 1,1,2-TCA. (C)2017 Elsevier B.V. All rights reserved.

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