Spatially-distinct redox conditions and degradation rates following field-scale bioaugmentation for RDX-contaminated groundwater remediation

JOURNAL OF HAZARDOUS MATERIALS

卷:387

Article

Michalsen, M. M.¹  King, A. S.²  Istok, J. D.³  Crocker, F. H.¹  Fuller, M. E.⁴  Kucharzyk, K. H.⁵  Gander, M. J.⁶ 

[1] US Army, Engineer Res & Dev Ctr, Environm Lab, Vicksburg, MS 39180 USA

[2] US Army, Corps Engineers, Seattle, WA 98134 USA

[3] Oregon State Univ, Sch Civil & Construct Engn, Corvallis, OR 97331 USA

[4] Aptim Fed Serv, Lawrenceville, NJ 08648 USA

[5] Battelle Mem Inst, 505 King Ave, Columbus, OH 43201 USA

[6] Naval Facil Engn Command, 1101 Tautog Circle, Silverdale, WA 98113 USA

关键词: RDX;    Gordonia KTR9;    Pseudomonas fluorescens I-C;    Bioaugmentation;    Groundwater;    Bioremediation;    Explosives;    Proteomics;   

DOI  :  10.1016/j.jhazmat.2019.121529

来源: Elsevier

【 摘 要 】

In situ bioaugmentation for cleanup of an hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX)-contaminated groundwater plume was recently demonstrated. Results of a forced-gradient, field-scale cell transport test with Gordonia sp. KTR9 and Pseudomonas fluorescens strain I-C cells (henceforth KTR9 and Strain I-C) showed these strains were transported 13 m downgradient over 1 month. Abundances of xplA and xenB genes, respective indicators of KTR9 and Strain I-C, approached injection well cell densities at 6 m downgradient, whereas gene abundances (and conservative tracer) had begun to increase at 13 m downgradient at test conclusion. In situ push-pull tests were subsequently completed to measure RDX degradation rates in the bioaugmented wells under ambient gradient conditions. Time-series monitoring of RDX, RDX end-products, conservative tracer, xplA and xenB gene copy numbers and XplA and XenB protein abundance were used to assess the efficacy of bioaugmentation and to estimate the apparent first-order RDX degradation rates during each test. A collective evaluation of redox conditions, RDX end-products, varied RDX degradation kinetics, and biomarkers indicated that Strain I-C and KTR9 rapidly degraded RDX. Results showed bioaugmentation is a viable technology for accelerating RDX cleanup in the demonstration site aquifer and may be applicable to other sites. Full-scale implementation considerations are discussed.

【 授权许可】

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