Mimicking the human respiratory system: Online in vitro cell exposure for toxicity assessment of welding fume aerosol

JOURNAL OF HAZARDOUS MATERIALS

卷:395

Article

Ward, Ryan X.^1,6  Tilly, Trevor B.¹  Mazhar, Syeda Irsa^1,2  Robinson, Sarah E.³  Eiguren-Fernandez, Arantzazu⁴  Wang, Jun⁵  Sabo-Attwood, Tara³  Wu, Chang-Yu¹ 

[1] Univ Florida, Dept Environm Engn Sci, Engn Sch Sustainable Infrastruct & Environm, 1128 Ctr Dr,220 Black Hall, Gainesville, FL 32611 USA

[2] Int Islamic Univ, Dept Environm Sci, Female Campus,Room 23,Hazrat Maryam Block,H-10, Islamabad, Pakistan

[3] Univ Florida, Coll Publ Hlth & Hlth Profess, Dept Environm & Global Hlth, HPNP 4157,1225 Ctr Dr,POB 100188, Gainesville, FL 32610 USA

[4] Aerosol Dynam Inc, 935 Grayson St, Berkeley, CA 94710 USA

[5] Univ Oklahoma, Dept Occupat & Environm Hlth, Hudson Coll Publ Hlth, Hlth Sci Ctr, 801 Northeast 13thSt, Oklahoma City, OK 73104 USA

[6] CALTECH, Div Geol & Planetary Sci, 1200 E Calif Blvd,Linde Robinson Lab MC 131-24, Pasadena, CA 91125 USA

关键词: Air-liquid interface;    Ultrafine particles;    Condensation;    Aerosol deposition;   

DOI  :  10.1016/j.jhazmat.2020.122687

来源: Elsevier

【 摘 要 】

In assessing the biological impact of airborne particles in vitro, air-liquid interface (ALI) exposure chambers are increasingly preferred over classical submerged exposure techniques, albeit historically limited by their inability to deliver sufficient aerosolized dose. A novel ALI system, the Dosimetric Aerosol in Vitro Inhalation Device (DAVID), bioinspired by the human respiratory system, uses water-based condensation for highly efficient aerosol deposition to ALI cell culture. Here, welding fumes (well-studied and inherently toxic ultrafine particles) were used to assess the ability of DAVID to generate toxicological responses between differing welding conditions. After fume exposure, ALI-cultured cells showed reductions in viability that were both distinct between welding conditions and linearly dose-dependent with respect to exposure time; comparatively, submerged cell cultures ran in parallel did not show these trends across exposure levels. DAVID delivers a substantial dose in minutes (> 100 mu g/cm(2)), making it preferable over previous ALI systems, which require hours of exposure to deliver sufficient dose, and over submerged techniques, which lack comparable physiological relevance. DAVID has the potential to provide the most accurate assessment of in vitro toxicity yet from the perspectives of physiological relevance to the human respiratory system and efficiency in collecting ultrafine aerosol common to hazardous exposure conditions.

【 授权许可】

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