The P Granules of C. elegans: A Genetic Model for the Study of RNA-Protein Condensates

JOURNAL OF MOLECULAR BIOLOGY

卷:430

Review

Seydoux, Geraldine¹ 

[1] Johns Hopkins Univ, Sch Med, Dept Mol Biol & Genet, HHMI, 725 N Wolfe St, Baltimore, MD 21205 USA

关键词: RNA granules;    P granules;    C. elegans;    Germline condensates;   

DOI  :  10.1016/j.jmb.2018.08.007

来源: Elsevier

【 摘 要 】

P granules are RNA/protein condensates in the germline of Caenorhabditis elegans. Genetic analyses have begun to identify the proteins that regulate P granule assembly in the cytoplasm of zygotes. Among them, the RGG-domain protein PGL-3, the intrinsically disordered protein MEG-3, and the RNA helicase LAF-1 all bind and phase separate with RNA in vitro. We discuss how RNA-induced phase separation, competition with other RNA-binding proteins, and reversible phosphorylation contribute to the asymmetric localization of P granules in the cytoplasm of newly fertilized embryos. P granules contain RNA silencing complexes that monitor the germline transcriptome and may provide an RNA memory of germline gene expression across generations. (C) 2018 Published by Elsevier Ltd.

【 授权许可】

Free