Novel LOTUS-domain proteins are organizational hubs that recruit C. elegans Vasa to germ granules

eLife

Michelle Gutwein¹  George Chung¹  Yu Guan¹  John Zinno¹  Olivia Bay¹  Hin Hark Gan¹  Fabio Piano²  Kristin C Gunsalus²  Patricia Giselle Cipriani²  Vinay K Mayya³  Thomas F Duchaine³  Jia-Xuan Chen⁴  Matthias Selbach⁴  Hala Fahs⁵ 

[1] Center for Genomics and Systems Biology, Department of Biology, New York University, New York, United States;Center for Genomics and Systems Biology, Department of Biology, New York University, New York, United States;NYU Abu Dhabi Center for Genomics and Systems Biology, New York University Abu Dhabi, Abu Dhabi, United Arab Emirates;Goodman Cancer Research Centre and Department of Biochemistry, McGill University, Montreal, Canada;Max Delbrück Center for Molecular Medicine, Berlin, Germany;NYU Abu Dhabi Center for Genomics and Systems Biology, New York University Abu Dhabi, Abu Dhabi, United Arab Emirates;

关键词: P granules;    germ granules;    IDR;    LOTUS;    vasa;    phase separation;    C. elegans;   

DOI  :  10.7554/eLife.60833

来源: eLife Sciences Publications, Ltd

【 摘 要 】

We describe MIP-1 and MIP-2, novel paralogous C. elegans germ granule components that interact with the intrinsically disordered MEG-3 protein. These proteins promote P granule condensation, form granules independently of MEG-3 in the postembryonic germ line, and balance each other in regulating P granule growth and localization. MIP-1 and MIP-2 each contain two LOTUS domains and intrinsically disordered regions and form homo- and heterodimers. They bind and anchor the Vasa homolog GLH-1 within P granules and are jointly required for coalescence of MEG-3, GLH-1, and PGL proteins. Animals lacking MIP-1 and MIP-2 show temperature-sensitive embryonic lethality, sterility, and mortal germ lines. Germline phenotypes include defects in stem cell self-renewal, meiotic progression, and gamete differentiation. We propose that these proteins serve as scaffolds and organizing centers for ribonucleoprotein networks within P granules that help recruit and balance essential RNA processing machinery to regulate key developmental transitions in the germ line.

【 授权许可】

CC BY