RNA Chaperones Stimulate Formation and Yield of the U3 snoRNA-Pre-rRNA Duplexes Needed for Eukaryotic Ribosome Biogenesis

JOURNAL OF MOLECULAR BIOLOGY

卷:390

Article

Gerczei, Timea^1,2  Shah, Binal N.¹  Manzo, Anthony J.³  Walter, Nils G.³  Correll, Carl C.¹ 

[1] Rosalind Franklin Univ Med & Sci, Dept Biochem & Mol Biol, N Chicago, IL 60064 USA

[2] Ball State Univ, Dept Chem, Muncie, IN 47306 USA

[3] Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA

关键词: annealing;    folding;    FRET;    fluorescence;    structural rearrangement;   

DOI  :  10.1016/j.jmb.2009.05.072

来源: Elsevier

【 摘 要 】

Short duplexes between the U3 small nucleolar RNA and the precursor ribosomal RNA must form quickly and with high yield to satisfy the high demand for ribosome synthesis in rapidly growing eukaryotic cells. These interactions, designated the U3-ETS (external transcribed spacer) and U3-18S duplexes, are essential to initiate the processing of small subunit ribosomal RNA. Previously, we showed that duplexes corresponding to those in Saccharomyces cerevisiae are only observed in vitro after addition of one of two proteins: Imp3p or Imp4p. Here, we used fluorescence-based and other in vitro assays to determine whether these proteins possess RNA chaperone activities and to assess whether these activities are sufficient to satisfy the duplex yield and rate requirements expected in vivo. Assembly of both proteins with the U3 small nucleolar RNA into a chaperone complex destabilizes a U3 stem structure, apparently to expose its 18S base-pairing site. As a result, the chaperone complex accelerates formation of the U3-18S duplex from an undetectable rate to one comparable with the intrinsic rate observed for hybridizing short duplexes. The chaperone complex also stabilizes the U3-ETS duplex by 2.7 kcal/mol. These chaperone activities provide high U3-ETS duplex yield and rapid U3-18S duplex formation over a broad concentration range to help ensure that the U3-precursor ribosomal RNA interactions limit neither ribosome biogenesis nor rapid cell growth. The thermodynamic and kinetic framework used is general and thus suitable for investigating the mechanism of action of other RNA chaperones. (C) 2009 Elsevier Ltd. All rights reserved.

【 授权许可】

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