Temporal Regulation of Gene Expression of the Escherichia coli Bacteriophage phiEco32

JOURNAL OF MOLECULAR BIOLOGY

卷:416

Article

Pavlova, Olga¹  Lavysh, Dania^2,3  Klimuk, Evgeny^2,3  Djordjevic, Marko⁴  Ravcheev, Dmitry A.^5,6  Gelfand, Mikhail S.^5,7  Severinov, Konstantin^1,2,3  Akulenko, Natalja^1,2,3 

[1] Waksman Inst Microbiol, Piscataway, NJ 08854 USA

[2] Russian Acad Sci, Inst Mol Genet, Moscow 123182, Russia

[3] Russian Acad Sci, Inst Gene Biol, Moscow 117312, Russia

[4] Univ Belgrade, Fac Biol, Inst Physiol & Biochem, Belgrade, Serbia

[5] Russian Acad Sci, Inst Informat Transmiss Problems, Moscow 127994, Russia

[6] Sanford Burnham Med Res Inst, La Jolla, CA 92037 USA

[7] Moscow MV Lomonosov State Univ, Fac Bioengn & Bioinformat, Moscow 119992, Russia

关键词: bacteriophage;    genome;    RNA polymerase;    sigma factor;    transcription regulation;   

DOI  :  10.1016/j.jmb.2012.01.002

来源: Elsevier

【 摘 要 】

Escherichia coli phage phiEco32 encodes two proteins that bind to host RNA polymerase (RNAP): gp79, a novel protein, and gp36, a distant homolog of sigma(70) family proteins. Here, we investigated the temporal pattern of phiEco32 and host gene expression during infection. Host transcription shutoff and three distinct bacteriophage temporal gene classes (early, middle, and late) were revealed. A combination of bioinformatic and biochemical approaches allowed identification of phage promoters recognized by a host RNAP holoenzyme containing the sigma(70) factor. These promoters are located upstream of early phage genes. A combination of macroarray data, primer extension, and in vitro transcription analyses allowed identification of six promoters recognized by an RNAP holoenzyme containing gp36. These promoters are characterized by a single-consensus element tAATGTAtA and are located upstream of the middle and late phage genes. Curiously, gp79, an inhibitor of host and early phage transcription by sigma(70) holoenzyme, activated transcription by the gp36 holoenzyme in vitro. (C) 2012 Elsevier Ltd. All rights reserved.

【 授权许可】

Free