The Xp10 Bacteriophage Protein P7 Inhibits Transcription by the Major and Major Variant Forms of the Host RNA Polymerase via a Common Mechanism

JOURNAL OF MOLECULAR BIOLOGY

卷:428

Article

Brown, D. R.¹  Sheppard, C. M.¹  Burchell, L.¹  Matthews, S.¹  Wigneshweraraj, S.¹ 

[1] Imperial Coll London, MRC Ctr Mol Microbiol & Infect, London SW7 2AZ, England

关键词: RNA polymerase;    sigma factor;    bacteriophage;    transcription regulation;    bacteria;   

DOI  :  10.1016/j.jmb.2016.08.004

来源: Elsevier

【 摘 要 】

The a factor is a functionally obligatory subunit of the bacterial transcription machinery, the RNA polymerase. Bacteriophage-encoded small proteins that either modulate or inhibit the bacterial RNAP to allow the temporal regulation of bacteriophage gene expression often target the activity of the major bacterial sigma factor, sigma(70). Previously, we showed that during Xanthomonas oryzae phage Xp10 infection, the phage protein P7 inhibits the host RNAP by preventing the productive engagement with the promoter and simultaneously displaces the sigma(70) factor from the RNAP. In this study, we demonstrate that P7 also inhibits the productive engagement of the bacterial RNAP containing the major variant bacterial sigma factor, sigma(54), with its cognate promoter. The results suggest for the first time that the major variant form of the host RNAP can also be targeted by bacteriophage-encoded transcription regulatory proteins. Since the major and major variant sigma factor interacting surfaces in the RNAP substantially overlap, but different regions of sigma(70) an and sigma(54) are used for binding to the RNAP, our results further underscore the importance of the sigma-RNAP interface in bacterial RNAP function and regulation and potentially for intervention by antibacterials. (C) 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

【 授权许可】

Free