Insights into the Distinct Mechanisms of Action of Taxane and Non-Taxane Microtubule Stabilizers from Cryo-EM Structures

JOURNAL OF MOLECULAR BIOLOGY

卷:429

Article

Kellogg, Elizabeth H.¹  Hejab, Nisreen M. A.²  Howes, Stuart³  Northcote, Peter⁴  Miller, John H.⁴  Fernando Diaz, J.⁵  Downing, Kenneth H.¹  Nogales, Eva^1,6,7,8 

[1] Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Grad Grp Comparat Biochem, Berkeley, CA 94720 USA

[3] Univ Calif Berkeley, Biophys Grad Grp, Berkeley, CA 94720 USA

[4] Victoria Univ Wellington, Ctr Biodiscovery, Wellington 6140, New Zealand

[5] CSIC, Ctr Invest Biol, Chem & Phys Biol, Plaza Murillo 2, E-28040 Madrid, Spain

[6] Univ Calif Berkeley, Inst QB3, Berkeley, CA 94720 USA

[7] Univ Calif Berkeley, Mol & Cell Biol Dept, Berkeley, CA 94720 USA

[8] Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA

关键词: cryo-EM;    microtubule;    microtubule-stabilizing agents;    Taxol;    peloruside;    zampanolide;   

DOI  :  10.1016/j.jmb.2017.01.001

来源: Elsevier

【 摘 要 】

A number of microtubule (MT)-stabilizing lagents (MSAs) have demonstrated or predicted potential as anticancer agents, but a detailed. structural basis for their mechanism of action is still lacking. We have obtained high-resolution (3.9-4.2 angstrom) cryo-electron microscopy (cryo-EM) reconstructions of MTs stabilized by the taxane-site binders Taxol and zampanolide, and by peloruside, which targets a distinct, non-taxoid pocket on 13-tubulin. We find that each molecule has unique distinct structural effects on the MT lattice structure. Peloruside acts primarily at lateral contacts and has an effect on the seam of heterologous interactions, enforcing a conformation more similar to that of homologous (i.e., non-seam) contacts by which it regularizes the MT lattice. In contrast, binding of either Taxol or zampanolide induces MT heterogeneity. In doubly bound MTs, peloruside overrides the heterogeneity induced by Taxol binding. Our structural analysis illustrates distinct mechanisms of these drugs for stabilizing the MT lattice and is of relevance to the possible use of combinations of MSAs to regulate MT activity and improve therapeutic potential. (C) 2017 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

【 授权许可】

Free